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Glycosaminoglycan antigens in peripheral nerve: Studies with antibodies from a patient with neuropathy and monoclonal gammopathy
Authors:Lorenza Freddo   William H. Sherman  Norman Latov  
Affiliation:1. Department of Neurology, Columbia University, College of Physicians and Surgeons, New York, NY USA;2. Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, NY U.S.A.;1. Innovation and Creativity Management, Department of Management Studies, NALSAR University of Law, Justice City, Shameerpet, Hyderabad, Telangana, 500101, India;2. Department of Information Management, Chaoyang University of Technology, Taichung, 413310, Taiwan;3. Department of Information Management, Chaoyang University of Technology, Wufeng, Taichung, 413310, Taiwan;4. School of Management Studies, University of Hyderabad, Telangana, 500046, India;5. Department of Strategic Management, Indian Institute of Management Kozhikode, India, 673570;6. Department of Commerce, Gurugram University, Gurugram, Haryana, 122413, India;7. Department of Commerce, Ramjas College, University of Delhi, New Delhi, 110007, India;8. NALSAR University of Law, Hyderabad, Telangana, 500101, India;1. Marine Biopolymers & Advanced Bioactive Materials Research Lab, Department of Prosthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600 077, Tamil Nadu, India;2. National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (Govt. of India), Chennai, 600 100, Tamil Nadu, India;3. Centre for Cellular and Molecular Research, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India;1. School of Civil Engineering, Dalian University of Technology (DUT), 2 Linggong Road, Dalian 116024, China;2. Zero Carbon Building Research Institute, Urban Development Design Group Co., Ltd., 186 Changchun Road, Dalian 116021, China
Abstract:An immunoblot staining procedure was developed for the detection of antibody binding to glycosaminoglycans (GAGs). The method was used to study the binding of a human monoclonal antibody (M-protein) from a patient with peripheral neuropathy, previously found to react with proteoglycans (PGs). GAGs prepared from human peripheral nerve were separated by electrophoresis on cellulose acetate membranes, transferred onto nitrocellulose sheets, and immunostained with the M-protein. The M-protein bound to a single GAG band with intermediate mobility which eluted with 1.25 N NaCl on ion-exchange chromatography and was chondroitinase sensitive. The M-protein appeared to bind to chondroitin sulfate containing proteoglycans in peripheral nerve.
Keywords:Antibodies   Antigens   Glycosaminoglycans   Monoclonal gammopathy   Neuropathy
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