温阳活血方对大鼠肾间质TIMP-1、MMP-3、FN的影响

目的观察温阳活血方对实验大鼠肾间质纤维化的保护作用并探讨其作用机理.方法实验采用单侧输尿管梗阻(UUO)大鼠模型,将其随机分为温阳活血组、福辛普利组、模型组,并设假手术组.观察梗阻侧肾脏组织形态学改变,免疫组化半定量分析各组的基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶抑制剂-1(TIMP-1)、纤维连接蛋白(FN)的表达水平并与假手术组比较.结果模型组及各治疗组与假手术组相比均呈不同程度的病理损害.模型组的MMP-3、TIMP-1、FN表达水平均高于假手术组(P＜0.05),且TIMP-1的表达明显高于MMP-3的表达.两个治疗组与模型组相比,MMP-3的表达水平高于模型组(P＜0.05);但TIMP-1、FN的表达水平低于模型组(P＜0.05);MMP-3、TIMP-1、FN在两个治疗组间的表达差异均无统计学意义(P＞0.05).结论温阳活血方可能通过抑制TIMP-1的表达,恢复MMP-3表达,以此来调节MMP-3/TIMP-1的比例,从而保护肾小管功能,减轻间质纤维化的进展.

Effect of Warming Yang and Activating Blood Formula on TIMP-1,MMP-3, FN in Rats with Renal Interstitial Fibrosis

to observe the protection of Warming Yang and Activating Blood Formula on renal interstitial fibrosis in rats and try to find the mechanism. Methods:The model of renal obstruction was established by unilateral ureteral obstruction (UUO) in rats, and then randomly divided into four groups, i.e. UUOW group (treated with the formula), UUOF group (treated with Fosinopril), UUO group and the sham-operation group. The morphological changes in obstructing renal tissue and the protein expressions of MMP-3, TIMP-1, FN were observed and compared. Results:Different degrees of kidney lesion were shown in UUO, UUOW, UUOF groups as compared with the SH group. The expressions of MMP-3, FN of UUO were higher than those of the SH group (P<0.05), and TIMP-1 was higher than MMP-3. Expressions of MMP-3 of both UUOF and UUOW had higher MMP-3 expression (P<0.05) and lower TIMP-1, FN expression than the UUO group(P<0.05). However, there were no significant difference among two treatment groups in all parameters (P>0.05). Conclusion:the formula may regulate the ratio between MMP-3/TIMP-1 by decreasing the expression of TIMP-1and increasing MMP-3, in that way protect renal tubule and delay the progression of renal interstitial fibrosis.