RP-HPLC法测定防风通圣丸中栀子苷、芍药苷、黄芩苷、大黄素的含量

目的:建立RP-HPLC同时测定防风通圣丸中栀子苷、芍药苷、黄芩苷和大黄素含量的方法。方法:采用高效液相色谱(RP-HPLC)梯度洗脱,在不同波长下测定含量。色谱柱:Zorbax Eclipse XDB-C18键合硅胶色谱柱(4.6 mm×150 mm,5μm);柱温为室温;流动相为乙腈-2%磷酸水溶液,采用梯度洗脱;流速1.0 m L/min;检测波长分别为240 nm(栀子苷、芍药苷),275 nm(黄芩苷、大黄素)。结果:栀子苷、芍药苷、黄芩苷和大黄素的进样量分别在0.056~0.56 g(r=0.999 7),0.103 5~1.029 7 g(r=0.999 2),0.207 4~3.128g(r=0.999 3),0.04~0.423g(r=0.999 6)范围内线性关系良好,平均加样回收率分别为99.14%,99.65%,99.03%,99.16%;RSD分别为1.08%,1.01%,0.85%,0.86%。结论:该方法简便、快速、灵敏、精密度高、重现性良好、结果准确,可用于同时测定防风通圣丸中栀子苷、芍药苷、黄芩苷和大黄素的含量。

Contents of Jasminoidin,Peoniflorin, Baicalin and Emodin in FangFeng TongSheng Pills Determined by RP-HPLC Method

Objective: To establish the determination methods for the contents of jasminoidin, peoniflorin, baicalin and emodin inFangFengTongShengpills simultaneously by RP-HPLC. Methods: The contents were deter-mined under different wavelengths by adopting RP-HPLC as gradient elution. Chromatographic column: Zorbax E-clipse XDB-C18 bonded silica gel column (4.6 mm×150 mm, 5μm); column temperature was room temperature; mo-bile phase: acetonitrile-2% phosphoric acid aqueous solution, gradient elution was adopted; flow rate 1.0mL/min; de-tection wavelengths were 240nm (jasminoidin and peoniflorin) and 275nm (baicalin and emodin) respectively. Re-sults: Jasminoidin, peoniflorin, baicalin and emodin were in better linear relationship when their sample sizes were between 0.056 and 0.56g (r=0.999 7), 0.103 5 and 1.029 7g(r=0.999 2), 0.207 4 and 3.128 g(r=0.999 3), 0.04 and 0.423 g(r=0.999 6), average recovery rates were 99.14%, 99.65%, 99.03% and 99.16%; RSD was 1.08%, 1.01%, 0.85% and 0.86% respectively. Conclusion: The method, simple, rapid, sensitive, reproducible and of high precision and accurate results, could be used for content determination of jasminoidin, peoniflorin, baicalin and emodin in FangFengTongShengpills simultaneously.