何首乌药效成分3种提取方法比较

目的:研究中药材何首乌的不同提取方法,比较所提取的成分,探索优化何首乌的提取工艺。方法:采用高效液相色谱法(HPLC)分析测定何首乌渗漉提取、75℃回流提取、89℃回流提取的提取液的二苯乙烯苷、蒽醌类化合物含量,并进行3种不同提取方法的提取液化学成分指纹图谱相似度及所得干物质考察。结果:3种提取方法提取的二苯乙烯苷、蒽醌类化合物的含量基本接近。结合蒽醌的含量89℃回流提取略高于渗漉提取;75℃回流提取略低于渗漉提取。何首乌3种不同提取方法制备的供试品溶液的特征指纹图谱与共有模式的相似度均大于0.95,符合国家对药材指纹图谱研究的技术要求(大于0.9)。结论:3种不同提取方法制备的指纹图谱相似程度高,其提取液化学成分差异不大。

Comparison of Three Extract Methods for the Medicinal Composition of HeShouWu

Objective: To study the different extract methods of herb HeShouWu' compare extracted components,and explore as well as optimize the extraction process of HeShouWu. Methods: The HPLC method was used to analyze and determine the contents of stilbene glycoside, anthraquinones in HeShouWu extract by percolation extraction, reflux extraction at the temperatures of 75℃and 89℃. The similarities and dry matters of the fingerprint of chemical components from extracts by three different extract methods were investigated. Results: The contents of stilbene glycoside and anthraquinones extracted by three extract methods were approximately equal. Contents of combined anthraquinones by 89℃reflux extraction were slightly superior to percolation extraction; Contents of combined anthraquinones by 75℃reflux extraction were slightly lower than percolation extraction. The characteristic fingerprint and similarity of common patterns of HeShouWu test solution prepared by three different extract methods were all greater than 0.95 which satisfied the technical requirements for the research of the fingerprint of Chinese medicinal herbs(> 0.9). Conclusion: The similarity degree of fingerprints prepared by three different extract methods is high and there is not an obvious difference in the chemical components from its extract.