HPLC法测定麦冬及环境中多效唑的残留量

目的:确立麦冬及其生长环境中多效唑残留的快速检测方法。方法:采用无水甲醇,超声30分钟提取麦冬样品及其生长环境中多效唑。采用Agilent-C18色谱柱,流动相为甲醇-乙腈-0.1%甲酸(50:15:35),流速1.0 m L/min;检测波长220 nm,柱温30℃。结果:在0.000 2~0.015μg之间线性良好,相关系数为0.999 6,回收率在98.6%~105.6%之间,RSD值为2.9149%,精密度实验、稳定性考察和重现性实验的RSD分别为0.998 5%、1.368 9%、1.008 3%。结论:本方法可快速、简便、准确地测定麦冬植株样品及其生长环境中的多效唑残留量。

Determination of Paclobutrazol Remained inMaiDongand Its Growth Environment by HPLC

Objective: To establish rapid determination method of paclobutrazol remained inMaiDong[Ophio-pogonjaponicus(Linn. f.) Ker-Gawl.]. Methods: Paclobutrazol remained inMaiDongwas extracted by ultrasound for 30 minutes in absolute methanol. Agilent-C18chromatographic column was adopted, mobile phase: methanol- ace-tonitrile-0.1% methanoic acid (50:15:35), flow rate: 1.0mL/min; detection wavelength was 220 nm, column tempera-ture: 30℃. Results: It showed better linear relationship between 0.000 2 and 0.015μg, correlation coefficient was 0.999 6, recovery rate was between 98.6% and 105.6%, RSD=2.9149%, RSD of precision, stability and reproducibility tests were 0.998 5%, 1.368 9% and 1.008 3%. Conclusion: The method could be used to determine residual quantity of paclobutrazol inMaiDongand its growth environment accurately, simply and rapidly.