针刀松解法对移植于裸鼠的人皮肤增生性瘢痕组织中成纤维细胞的影响

目的观察针刀松解法对移植于裸鼠皮下人增生性瘢痕成纤维细胞增殖细胞核抗原(proliferation cell nuclearantigen,PCNA)、Ⅰ、Ⅲ型胶原、bcl-2和bax的作用,探讨其对移植于裸鼠皮下的人增生性瘢痕组织中成纤维细胞生物学特性的影响。方法取6例人增生性瘢痕组织,削去瘢痕表皮及皮下组织,将每例增生性瘢痕组织分成重约0.2 g的3块,每只裸鼠移植1块,共移植于18只裸鼠背部皮下,建立增生性瘢痕裸鼠动物模型。移植后10 d在瘢痕组织内分3点分别注射生理盐水0.1 ml(对照组)、注射0.1 mg/ml曲安奈德0.1 ml(曲安奈德组)、将小针刀刺进瘢痕组织内,在瘢痕内向四周切割剥离至瘢痕周边(针刀松解组)进行治疗,每组6只。治疗后14 d取材,利用HE染色计数分析各组成纤维细胞的含量;免疫组织化学染色检测各组PCNA、Ⅰ、Ⅲ型胶原、bcl-2和bax表达阳性成纤维细胞数的变化。结果 HE染色结果:与对照组成纤维细胞数量(913.33±148.95)个/mm2相比,曲安奈德组(853.33±62.82)个/mm2和针刀松解组(863.33±75.28)个/mm2均降低,但是差异无显著性(P>0.05)。免疫组织化学染色结果:曲安奈德组和针刀松解组PCNA、Ⅰ、Ⅲ型胶原蛋白阳性的成纤维细胞数量减少,与对照组相比差异有统计学意义(P<0.05);3组bcl-2和bax阳性的成纤维细胞数量以及bcl-2/bax比率差异无显著性(P>0.05)。结论 针刀松解法具有抑制移植于裸鼠皮下人增生性瘢痕成纤维细胞的增殖和分泌合成的能力;针刀松解对增生性瘢痕成纤维细胞中bcl-2和bax表达及其比率没有影响。

Effect of Small Needle-knife Therapy on the Fibroblasts in Human Hypertrophic Scar Tissues Transplanted into Nude Mice

Objective To discuss the effect of small needle-knife therapy on the biological characteristics of fibroblasts of the hypertrophic scar tissues subcutaneously transplanted into nude mice by detecting the expressions of PCNA,collagen typeⅠ and Ⅲ,bcl-2 and bax in the fibroblasts.Methods To establish the animal model of hypertrophic scar on nude mice,6 samples of hypertrophic scar tissues without cuticle were respectively divided into three pieces and transplanted subcutaneously into the back of 18 nude mice.Ten days after the operation,the mice were treated with saline(control group),0.1 mg/ml triamcinolone(triamcinolone group),or small needle-knife(small needle-knife group),respectively(n=6 in each).After 14-day treatment,pathological changes and the number of the fibroblasts positively expressed PCNA,Ⅰcollagen,Ⅲ collagen,bcl-2 and bax in the hypertrophic scar tissues were detected by HE staining and immunohistochemistry. Results HE staining showed that,compared with the control group(913.33±148.95)/mm2,the number of fibroblast were reduced in the triamcinolone(853.33±62.82)/mm2 and small needle-knife groups(863.33±75.28)/mm2,but no significant difference was found among the groups(P>0.05).Immunohistochemistry indicated that,in the triamcinolone and small needle-knife groups,the number of fibroblast that were positively stained with PCNA,and Ⅰ and Ⅲ collagen were significantly lower than those in the control(P<0.05),while the number and the bcl-2/bax ratios of the fibroblast positively stained with bcl-2 and bax were not significant different among the three groups(P>0.05).ConclusionsSmall needle-knife therapy can inhibit the proliferating,synthesizing and secreting abilities of the fibroblasts in hypertrophic scar tissues transplanted into nude mice.It has no influence on the expressions of bcl-2 and bax in the fibroblasts in human hypertrophic scar tissues,nor on the bcl-2/bax ratios.