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| 大鼠鼠胚海马神经细胞原代培养及神经元鉴定 | |
| 引用本文： | 杨明峰,岳伟,张伟,张颜波,孙保亮,牛敬忠.大鼠鼠胚海马神经细胞原代培养及神经元鉴定[J].泰山医学院学报,2006,27(7):614-616. |
| 作者姓名： | 杨明峰 岳伟 张伟 张颜波 孙保亮 牛敬忠 |
| 作者单位： | 1. 泰山医学院生命科学研究中心,山东,泰安,271000 2. 泰山医学院附属医院,山东,泰安,271000 3. 菏泽医学高等专科学校,山东,菏泽,274030 |
| 摘 要： | 目的建立大鼠鼠胚海马神经元原代培养的方法。方法取孕龄17-18天的Wistar大鼠腹中胎鼠,血清培养法进行海马神经元的原代培养,然后用倒置显微镜进行形态学观察和神经元特异性烯醇化酶免疫组织化学方法进行神经元的鉴定。结果培养8天后的海马神经元经形态学和组织化学方法观察证实神经细胞发育成熟。结论大鼠鼠胚海马神经元原代培养是一种新型的培养方法,具有明显的优势。 |
| 关 键 词： | 大鼠 海马 神经元 细胞培养 |
| 文章编号： | 1004-7115（2006）07-0614-03 |
| 收稿时间： | 2006-08-20 |
| 修稿时间： | 2006年8月20日 |
Primary culture method for hippocampal neurons of embryo of rat and identification of neurons |
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| YANG Ming-feng,YUE Wei,ZHANG Wei,ZHANG Yan-bo,SUN Bao-liang,NIU Jin-zhong.Primary culture method for hippocampal neurons of embryo of rat and identification of neurons[J].Journal of Taishan Medical College,2006,27(7):614-616. | |
| Authors: | YANG Ming-feng YUE Wei ZHANG Wei ZHANG Yan-bo SUN Bao-liang NIU Jin-zhong |
| Institution: | 1. Reseach Central of Life Science, Taishan Medical College, Taian 27100, China;2. Dept. of Haematology, Affiliated Hospital of Taishan Medical College, Taian 27100, China; 3. Heze Medical College, Heze 274030, China |
| Abstract: | Objective: To establish primary culture method for hippocampal neurons of embryo of rat.Methods: Embryo of rat which was pregnant for 17-18 days was used in experiment.Hippocampal neurons were cultured with serum combination.Cultured neurons were observed by inverted microscope and identificated by the method of NSE.Results: Hippocampal neurons were founded to be maturated by the methods of morphology and immunohistochemistry.Conclusions: Primary culture method for hippocampal neurons of embryo of rat is a new method of culture,which is proved to outweigh other methods. |
| Keywords: | rat hippocampus neuron cell culture |
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