Sequence relations and coding properties of a subgenomic RNA isolated from barley stripe mosaic virus |
| |
Authors: | Jackson A O Dawson J R Covey S N Hull R Davies J W McFarland J E Gustafson G D |
| |
Affiliation: | Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, United Kingdom. |
| |
Abstract: | A subgenomic (SG) RNA ( approximately 800 nucleotides) is a minor component of barley stripe mosaic virus RNAs. The SG-RNAs isolated from the Type and North Dakota 18 (ND18) strains of BSMV have sequence homology with RNA 2 of the ("pseudo-two component") Type strain, which has two electrophoretic components, but only limited homology is evident with RNA 2 of the ND18 and Norwich strains, which have three electrophoretic components ("three component" strain). Instead, eDNAs from SG-RNA hybridize most efficiently with RNA 3 of the ND18 and Norwich strains. In wheat germ extracts the SG-RNAs direct the synthesis of two polypeptides with apparent molecular weights of 20 to 21 x 10(3). However, these two polypeptides were difficult to detect by polyacrylamide gel electrophoresis of extracts from Type- or ND18-infected barley and so appear not to accumulate during infection. |
| |
Keywords: | To whom reprint requests should be addressed. |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|