促红细胞生成素对脊髓缺血再灌注损伤保护作用及其机理的实验研究

目的:探讨促红细胞生成素(EPO)处理对脊髓缺血再灌注损伤的保护作用及其可能的作用机理.方法:建立兔脊髓缺血再灌注损伤模型,通过动物神经运动功能评分和组织学染色评价神经损伤程度;采用免疫组织化学染色测量bcl-2和bax蛋白表达情况;TUNEL法检测脊髓细胞凋亡情况.结果:EPO处理组能明显改善动物的神经运动功能并减轻脊髓由于缺血再灌注所引起的病理性损害:EPO组与对照组相比,脊髓前角细胞的凋亡指数明显下降(P＜0.05),脊髓内bcl-2表达增高(P＜0.01),而bax蛋白表达下降(P＜0.01).结论:EPO对脊髓由于缺血而引起的神经运动功能损害具有保护作用,此种保护作用与EPO能够上调bcl-2和下调bax蛋白表达有关.

The protective mechanism of erythropoietin on spinal cord injury resulted from ischemia-reperfusion in rabbits

Objective:To investigate the protective effects of erythropoietin (EPO)treatment on spinal cord injury resulted from ischemia-reperfusion and to explore its possible mechanism.Method:The spinal cord ischemia-reprefusion model were made by occluding infrarenal abdominal aorta below the origin of the erenal artery for 20 minutes in rabbits.The injuries of spinal cord were estimated by the scores of neurological function and histopathology.The immunohistochemistry and TUNEL assay were still used in this study.Result:The neurological function was improved significantly after treatment with EPO and EPO could attenuate the pathologic injury due to spinal cord ischemic-reperfusion,The apoptosis index of the spinal anterior horn cells in the EPO group was decreased significantly in control group (P<0.01).The expression of bc 1~2 protein was increased (P<0.01)while the expression of the bax protein was decreased (P<0.01)in EPO group.Conclusion:The neurrological functions and pathologic expression on spinal cord injury resulted from ischemia-reprefusion are attenuated significantly after treatment with EPO,The mechanism is possibly related to the expressions of bcl-2 and bax proteins.