Characterization of 5-HT Receptors in Rat Proximal Colon |
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| Institution: | 1. Dokuz Eylul University Medical Faculty, Department of Pharmacology, 35340 Balçova, Izmir, Turkey [Tel: 90-232-2777333;1. School of Mechanical and Energy Engineering, Tongji University, Jiading District, Shanghai 201804, China;2. Construction and Development Corporation of Chenjia Town, Chongming County, Shanghai 202162, China;1. ISAE-SUPAERO, Université de Toulouse, 10 avenue Edouard Belin, 31055, Toulouse, France;2. Department of Applied Psychology, Cardiff Metropolitan University, Cardiff, CF5 2YB, UK;3. École de psychologie, Université Laval, Québec, QC, G1V 0A6, Canada;4. School of Information Technology and Electrical Engineering, University of Queensland, Brisbane, QLD 4072, Australia;5. Laboratoire de Neurosciences Cognitives, Inserm unit 960, Département d’Etudes Cognitives, Ecole Normale Supérieure, PSL Research University, 75005, Paris, France;1. Oregon State University, Department of Public Health, 123 Women’s Building, Corvallis, OR 97321, United States;2. Pacific Lutheran University, 12180 Park Ave S., Tacoma, WA 98447, United States;4. Oregon State University, Department of Public Health, 123 Women’s Building, Corvallis, OR 97321, United States;3. Cell Growth and Metabolism Section, Diabetes, Endocrinology, and Obesity Branch, NIDDK, National Institutes of Health, Clinical Research Center, Bethesda, Maryland 20892;5. Mouse Metabolism Core Laboratory, NIDDK, National Institutes of Health, Clinical Research Center, Bethesda, Maryland 20892;4. Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Wisconsin, Madison, Wisconsin 53705;6. Institute of Molecular and Cell Biology (IMCB), A*STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos#3-09, Singapore 138673, Singapore;12. Department of Pharmacology and Brehm Center for Diabetes Research, University of Michigan Medical School, Ann Arbor, Michigan 48105 |
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| Abstract: | - 1.In view of its multiple sites of action, we investigated the activity of 5-hydroxytryptamine (5-HT, serotonin) on various potential receptors in the isolated proximal colon of rats.
- 2.5-HT induced concentration-dependent contractions of colonic strips (pEC50=7.54±0.12).
- 3.The 5-HT1 receptor agonist, 5-carboxamidotryptamine, induced concentration-dependent contractions (pEC50=5.93±0.27); however, neither the 5-HT3 receptor-agonist, phenylbiguanide, nor the 5-HT4 receptor-agonist, renzapride, caused contractions at concentrations as high as 10?4 M.
- 4.The 5-HT1/2 receptor antagonist, methiothepin, caused concentraction-dependent nonsurmountable antagonism. The 5-HT3 receptor antagonist, tropisetron, inhibited the contractions to a concentration of 5-HT?10?6 M. Ketanserin had no effect on responses to 5-HT.
- 5.Tetrodotoxin and atropine had no effect on responses to 5-HT.
- 6.We conclude that contractions to 5-HT are mediated by 5-HT1-like and probably 5-HT3 receptors that activate tetrodotoxin insensitive mechanisms.
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