The morphogenesis of mouse vallate gustatory epithelium and taste buds requires BDNF-dependent taste neurons |
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| Institution: | 1. Image Sciences Institute, University Medical Center Utrecht Brain Center, Utrecht University, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands;2. The Rowett Institute, University of Aberdeen, Foresterhill Road AB25 2ZD, Scotland;3. Division of Biomedical Sciences, Centre for Health Science, University of the Highlands and Islands, Inverness IV2 3JH, United Kingdom;4. Department of Nutrition-Dietetics, School of Health Science & Education, Harokopio University Athens, 70 El. Venizelou avenue, 17671 Kallithea, Greece;5. Department of Nutrition and Dietetics, School of Physical Education, Sport Science and Dietetics, University of Thessaly, Trikala 42132, Greece;6. Department of Clinical Biochemistry, Cambridge University Hospitals, Cambridge, United Kingdom;7. Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Hills Rd, Cambridge CB2 0QQ, United Kingdom;8. NNF Center for Basic Metabolic Research and Research Section, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;9. Division of Human Nutrition and Health, Wageningen University, Stippeneng 4, 6708 WE, Wageningen, the Netherlands;1. Department of Psychology, Centre for Cognitive Neuroscience, Paris-Lodron-University of Salzburg, Hellbrunner Str. 34, 5020 Salzburg, Austria;2. Department of Psychology, Division of Clinical Psychology and Psychopathology, Paris-Lodron-University of Salzburg, Hellbrunner Str. 34, 15020 Salzburg, Austria;1. Department of Biomedical Science, Graduate School, Kyungpook National University, Daegu, Republic of Korea;2. Leading-Edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University, Daegu, Republic of Korea;3. Research Institutes of Aging and Metabolism, Kyungpook National University, Daegu, Republic of Korea;4. Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea;5. BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University, Daegu, Republic of Korea |
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| Abstract: | The developmental absence of brain-derived neurotrophic factor (BDNF) in null mutant mice caused three interrelated defects in the vallate gustatory papilla: sparse innervation, a reduction in the area of the gustatory epithelium, and fewer taste buds. On postnatal day 7, the stunted vallate papilla of bdnf null mutant mice was 30% narrower, the trench walls 35% reduced in area, and the taste buds 75% less abundant compared with wild-type controls. Quantitative assessment of innervation density was carried out to determine if the small trench walls and shortage of taste buds could be secondary consequences of the depletion of gustatory neurons. The diminished gustatory innervation was linearly associated with a reduced trench wall area (r=+0.94) and fewer taste buds (r=+0.96). Residual taste buds were smaller than normal and were innervated by a few surviving taste neurons. We conclude that BDNF-dependent taste neurons contribute to the morphogenesis of lingual gustatory epithelia and are necessary for both prenatal and postnatal mammalian taste bud formation. The gustatory system provides a conspicuous example of impaired sense organ morphogenesis that is secondary to sensory neuron depletion by neurotrophin gene null mutation. |
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