Regional differences in the nitrergic innervation between the proximal and the distal colon in rats |
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| Affiliation: | 1. Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università di Palermo, Via Archirafi 32, 90123, Palermo, Italy;2. Humanitas Clinical and Research Center, Via Manzoni 113, 20089, Rozzano, MI, Italy;3. SiSaf Ltd, Innovation Centre, Northern Ireland Science Park, Queen''s Island, Belfast, BT3 9DT, UK;1. School of Nursing, Division of Geriatrics, Gerontology, and Palliative Care, University of Alabama at Birmingham, NB 2M019C, 1701 University Boulevard, Birmingham, AL 35233, USA;2. Division of Medical Oncology, Duke Palliative Care, Duke Clinical Research Institute, Duke University, 2400 Pratt Street, #8043, Durham, NC 27710, USA;3. Division of Pulmonary Diseases and Critical Care Medicine, University of North Carolina – Chapel Hill, Bioinformatics Building, Suite 4124, Campus Box 7020, Chapel Hill, NC 27599, USA;1. Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, Shanghai, China;2. Department of Anesthesiology, Chenggong Hospital, Xiamen University, Xiamen, China;3. Department of Anesthesiology, 285th Hospital of CPLA, Handan, China;4. Department of Anesthesiology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China;1. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA;2. Departments of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA |
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| Abstract: | Background & Aims: Functional differences in the inhibitory neural pathway between the proximal and the distal colon are unknown. Methods: We investigated the nonadrenergic, noncholinergic (NANC) relaxation, nitric oxide synthase (NOS) synthesis, and NOS messenger RNA (mRNA) expression of the myenteric plexus in the proximal and the distal colon in rats. Results: Transmural nerve stimulation of the neuromuscular preparations from the proximal colon showed greater NANC relaxations than those from the distal colon. NANC relaxations were abolished by the NO biosynthesis inhibitor (NG-nitro-L-arginine methyl ester) in the proximal and the distal colon, suggesting mediation by NO released from the myenteric plexus. The average number of NOS-immunoreactive cells was significantly higher in the tissue from the proximal colon than in the tissue from the distal colon. Western and Northern blot analyses showed a higher density of the immunoreactive NOS band and the NOS mRNA band in the tissue from the proximal colon than in that from the distal colon. Conclusions: These observations indicate that the number of NOS-containing neurons and the NOS activity are increased in the myenteric plexus of the proximal colon compared with the distal colon, resulting in greater NANC relaxation in the proximal colon. These findings may explain the physiological role of the proximal colon as an organ for fecal storage and absorption of excess fluid.GASTROENTEROLOGY 1998;115:1504-1512 |
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