Effect of tumor-associated macrophages on gastric cancer stem cell in omental milky spots and lymph node micrometastasis |
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| Authors: | Chi Zhang Xiang Hu Xiao-Yu Liu Pin Liang Jian Zhang Liang Cao Zheng-Lin Wang Huan-Ran Liu Xun-Guo Yin Cheng-Yong Dong Li-Ming Wang |
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| Affiliation: | 1Department of Gastrointestinal Surgery, The First Affiliated Hospital of Dalian Medical Universiey, Dalian, China;2Department of Hepatobiliary Pancreas Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, China;3Liaoning Laboratory of Cancer Genetics and Epigenetics, Department of Cell Biology, Dalian Medical University, Dalian, China |
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| Abstract: | We observed whether the effect of tumor-associated macrophages on gastric cancer stem cell in omental milky spots and lymph nodes micrometastasis and research its possible mechanism. Macrophage THP-1 cells and Human gastric adenocarcinoma SGC-7901 cells were collectively cultivated in vivo. We found macrophage could suppress the proliferation and accelerated cell death of MFC cell. Meanwhile, these effects may be concerned with many signaling pathways, and we detected MCP-1 and COX-2 miRNA expressions, PGE-2 release levels, IL-4 and IL-10 activities, and TGF-β, IFN-γ, VEGF, MMP-2 and MMP-9 protein expressions in collectively cultivated cell. We found that MCP-1 and COX-2 miRNA expressions, and PGE-2 release levels were suppressed, IL-4 activity was inhibited and IL-10 activity was activated in collectively cultivated cell. Meanwhile, TGF-β, MMP-2 and MMP-9 protein expressions were inhibited and IFN-γ and VEGF protein expressions were activated in collectively cultivated cell. Taken together, these results suggest that the effect of tumor-associated macrophages on gastric cancer stem cell in omental milky spots and lymph nodes micrometastasis via COX-2/PGE-2/TGF-β/VEGF signal pathways. |
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| Keywords: | Tumor-associated macrophages gastric cancer stem cell COX-2 VEGF |
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