原人参三醇组皂苷和人参皂苷-Re对MPP＋诱导的SH-SY5Y细胞损伤的保护作用

目的：探讨原人参三醇组皂苷（protopanaxatriol group，PPT）和人参皂苷-Re对1-甲基-4-苯基-吡啶阳离子（1-methyl-4-phenylpyridiniumion，MPP＋）诱导的人多巴胺能神经母细胞瘤系SH-SY5Y细胞损伤的保护作用。方法：用不同浓度的PPT和人参皂苷-Re预处理细胞，以及不同体积分数的PPT和人参皂苷-Re含药血清预处理细胞，采用噻唑蓝（methylthiazolyldiphenyl-tetrazolium bromide，MTT）法检测细胞活力，计算细胞存活率，比较各组间的差异。结果：5μmol·L^-1和10μmol·L^-1的PPT和人参皂苷-Re均可以显著增加细胞存活率（与模型组比较，P〈0．05），体积分数10％的PPT和人参皂苷-Re含药血清能显著增加细胞存活率（与模型组比较，P〈0．001）。结论：PPT和人参皂苷-Re对MPP＋诱导的SH-SY5Y细胞损伤有显著的保护作用，具有潜在的抗帕金森病作用。

Protective Effect of PPT and Ginsenoside-Re in MPP+-treated SH-SY5Y Cells

Objective：To investigate the protective effect of PPT （protopanaxatriol group） and ginsenoside-Re on l-methyl-4-phenylpyridiniumion （MPP＋） -induced damage in SH- SY5Y （human dopaminergic neuroblastoma cell line） cells. Metbods：SH-SY5Y cells were treated with different concentrations of PPT,ginsenoside-Re or MPP containing serum. MTT colorimetry was used to test the viability of the cells and the survival rates were calculated and analyzed. Results：Cells treated by either 5 μmol·L^-1, 10 μmol·L^-1 PPT or ginsenoside-Re showed significantly higher viability as compared to control group （P〈0.05）. In addition, 10% PPT treated or ginsenoside-Re medicated serum treated groups showed similar protective effect.Conelusion.PPT and ginsenoside-Re have neuroprotective effects in a cell model of Parkinson＇s disease MPP~ -induced SH-SYSY cell death, suggesting that both PPT and ginsenoside-Re may have anti-PD effect.