几种适合药物筛选的抗氧化实验微孔板测定方法及其稳定性研究

目的：建立一系列适用于药物筛选的微孔板抗氧化实验方法。方法：采用SpectraMax M5连续光谱酶标测试仪,建立抗DPPH氧化活性、清除羟自由基实验方法（邻二氮菲-Fe2＋氧化法）、还原能力测定及抗脂质过氧化微孔板抗氧化实验方法。结果：这四种抗氧化微孔板实验方法具有稳定性好,所需试剂种类少、耗材量少,方法简便及可重复性强等共同特点;相对而言,抗DPPH氧化和还原实验两种实验方法在这几方面更具优势,更适合于作为抗氧化药物的大规模初筛基础试验方法。结论：这四种微孔板抗氧化实验方法,可以广泛应用于药物筛选,特别是高通量药物筛选研究。

Establishment about Several Anti-oxidant Models of Microplate Quanti-fication Suitable for Drug-screening and Their StabilityTesting

Objective： To establish a series of anti-oxidant models ofmicroplate quantification suitable for the drug screening. Methods： By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector, the anti-oxidant microplate quantification experiment modles of anti-DPPH oxidation activity, elimination hydroxy free radical, reduction ability determination, and lipid peroxidation were established. Results：All of the anti-oxidant microplate quantification experiment modles had more common characteristices of the good stability, the fewer reagent types and quantity, simple operation and step, the good repeatability, and so on. Relatively speaking, the two anti-oxidant modles of anti-DPPH oxidation activity, reduction ability determination had more advantage in these experiment modles, and had more suitable for initially screening experiment of the anti-oxidant drug as basic research. Conclusion： These four modles of the anti-oxidant microplate quantification may be widely applied in anti- drug screening, especially high-throughput screening research.