蜂胶提取物制备工艺研究

目的 优化蜂胶提取物制备工艺。方法 以浸膏得率，总黄酮、白杨素、乔松素和高良姜素质量分数为指标，采用单因素和正交试验，比较不同提取方法、提取溶剂、提取次数、提取时间、溶剂用量对蜂胶提取物制备工艺的影响。结果 蜂胶提取物最佳工艺：蜂胶冷藏后粉碎，加5倍量95%乙醇浸渍（加搅拌）提取4 h，滤过，回收乙醇，浓缩、干燥。结论 优化后的蜂胶提取物制备工艺简单、合理，适于进一步推广应用。

Preparation technology of propolis extract

Objective To establish the preparation technology of propolis extract. Methods With the contents of total flavonoids, chrysin, pinocembrin, and galangin and extract yield as evaluation indexes, the extraction methods, extraction solvent, extraction times, extraction time, and amount of solvent extraction were optimized with single factor test and orthogonal design to investigate the effect on the preparation technology of propolis extract. Results The optimal extraction technology was that propolis was refrigerated, grinded, soaked with 95% five times the amount of ethanol, shaked, then extracted for 4 h, filtrated, concentrated, and dried after ethanol recovery. Conclusion The optimial extracting technology of propolis is stable and feasible, and suitable for further application.