山楂总黄酮对酒精诱导小鼠酒精性肝病的影响

目的 研究山楂总黄酮对酒精诱导小鼠酒精性肝病的影响.方法 以56％体积分数白酒（1.5ml·100g-1）体重造模6周后,随机分组,山楂黄酮低、中、高剂量组（5,10,20g·kg1）和联苯双酯组（0.6g·kg1）,在酒精继续灌胃的基础上,按剂量给药,1次·d-1,共12周.检测小鼠血清和肝组织中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、超氧化物歧化酶（SOD）活性及丙二醛（MDA）、一氧化氮（NO）、谷胱甘肽过氧化物酶（GSH-PX）、肝匀浆中肿瘤坏死因子（TNF-α）含量.结果 山楂总黄酮对酒精诱导小鼠酒精性肝病形成后SOD、GSH-PX的活性含量有回升作用,并能阻止与肝病相应的ALT、AST活性及降低MDA、NO、TNF-a含量.结论 山楂总黄酮对酒精诱导小鼠酒精性肝病有预防作用,可能与其抗氧化和免疫调节作用有关.

Effect of Total Flavone of Hawthorn on Ethanol Induced Alcoholic Liver Disease in Mice

Objective To study the effect of total flavonoids of hawthorn on ethanol induced alcoholic liver disease in mice. Methods Animal model was established by 56% （v/v） （1.5ml · 100^-1 ） body,which were divided hawthorn flavonoids of low, middle and high dose group,（5, 10, 20g · kg^-1 ） and bifendate group （0. 6g · kg ^-1 ） after 6 weeks. Every group was respectively treated for 12 weeks with total flavonoids of hawthorn, bifendate or normal saline accordingly. The activities of alanine transaminase （ALT） aspartate transaminase （AST） in serum , superoxide dismutase （ SOD） ,malondialdehyde （MDA） ,nitric oxide （NO） ,glutathione peroxidase （GSI-t-PX） in liver homogenate, tumor necrosis factor （TNF-a） content were investigated. Results otal flavone of hawthorn can increase activity of SOD,GSH-PX,diseaseing ALT,AST activity and MDA,NO,TNF a content. Conclusion Hawthorn flavonoids have a preventive effect on alcoholic liver disease in mice induced by alcohol, which may be related to its antioxidant and immune regulation