盐析法制备小檗碱壳聚糖纳米载药微球

目的：探索制备小檗碱壳聚糖载药纳米微球颗粒的新方法及关键技术。方法：采用盐析法，通过三聚磷酸钠交联制备小檗碱壳聚糖纳米载药微球，测定载药微球的包封率，观察其扫描电镜图及红外光谱。结果：小檗碱颗粒粒径分布于400~500 nm，经壳聚糖包裹的小檗碱纳米颗粒形状规则，具有核-壳结构，平均粒径约500 nm，包封率约70%。结论：壳聚糖包裹的小檗碱颗粒具有均一的纳米粒径和良好的包封率，为小檗碱缓释用药和靶向给药设计提供参考。

Preparation of Berberine Loaded Chitosan Nanoparticles by Salt Precipitation

Objective: To explore new methodsand key technologies for preparation of berberine drug-loaded chitosan microspheres nanoparticles. Method: Salt precipitation was adopted,berberine loaded chitosan nanoparticles were prepared by sodiumtripolyphosphate crosslinking method,then encapsulation efficiency of drug-loaded microspheres was measured,load capability of nanopaticles was measured by ultraviolet-visible spectrum and infrared spectrum,while its morphology was tested by scanning electron microscope and particle-size analyzer. Result: Particle size distribution of berberine particles was 400-500 nm,the average diameter of berberine loaded chitosan nanoparticles was about 500nm with core-shell structure in regular shape,encapsulation efficiency was about 70%. Conclusion: Berberine loaded chitosan nanoparticles had a uniform particle size and good encapsulation effciency,this study could provide a reference for release medication and targeted drug design of berberine.