| 褪黑素对间歇低氧大鼠海马损伤保护作用的研究 |
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| 引用本文： | 李光,柴文戍,康健.褪黑素对间歇低氧大鼠海马损伤保护作用的研究[J].中国全科医学,2011,14(21). |
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| 作者姓名： | 李光 柴文戍 康健 |
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| 作者单位： | 1. 辽宁医学院附属第一医院呼吸科,辽宁省锦州市,121001
2. 中国医科大学附属第一医院 |
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| 摘 要： | 目的 探讨褪黑素(melatonin,MEL)对间歇低氧(intermittent hypoxia,IH)大鼠海马损伤的保护作用.方法 30只成年雄性Sprague-Dawley大鼠随机分成对照组(CON组)、间歇低氧组(IH组)、MEL治疗组(MEL组),每组10只.采用化学比色法测定海马组织丙二醛(MDA)、超氧化物歧化酶(SOD)水平,并采取RT-PCR方法检测海马组织中铜/锌超氧化物歧化酶(Cu/ZnSOD)、谷胱甘肽过氧化物酶(GPx)、组织过氧化氢酶(CAT)的mRNA水平.结果 IH组MDA水平显著高于对照组,SOD活性、Cu/ZnSOD、GPx、CAT的mRNA水平显著低于对照组;MEL组MDA水平明显低于IH组,SOD活性、Cu/ZnSOD、GPx、CAT的mRNA水平明显高于IH组.结论 褪黑素能抑制间歇低氧导致的氧化应激,从而对间歇低氧大鼠海马损伤具有保护作用.
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| 关 键 词： | 褪黑素 间歇低氧 氧化应激 |
Study on the Protective Effect of Melatonin against Intermittent Hypoxia Induced Hippocampal Injury in Rats |
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| LI Guang,CHAI Wen-shu,KANG Jian.Study on the Protective Effect of Melatonin against Intermittent Hypoxia Induced Hippocampal Injury in Rats[J].Chinese General Practice,2011,14(21). |
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| Authors: | LI Guang CHAI Wen-shu KANG Jian |
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| Institution: | LI Guang,CHAI Wen-shu,KANG Jian.Department of Respiratory Deseases,the First Affiliated Hospital of Liaoning Medical College,Jinzhou 121001,China |
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| Abstract: | Objective To explore the protective effect of melatonin(MEL) against intermittent hypoxia induced hippocampal injury in rats.Methods 30 adult male Sprague-Dawley rats were randomly divided into control group,IH group and MEL therapeutic group,with each group 10 rats.The levels of MDA and SOD were detected by colorimetric method,and RT-PCT was used to detect mRNA levels of the Cu/ZnSOD,GPx and CAT in hippocampal tissues.Results The level of MDA in IH group were significantly higher than that in control group... |
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| Keywords: | Melatonin Intermittent hypoxia Oxidative stress |
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