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| CD147 RNA干扰对前列腺癌细胞生长的影响 | |
| 引用本文： | 方芳,方青,鞠晓红,钟月,王立国.CD147 RNA干扰对前列腺癌细胞生长的影响[J].中国现代医学杂志,2007,17(14):1720-1722. |
| 作者姓名： | 方芳 方青 鞠晓红 钟月 王立国 |
| 作者单位： | 1. 吉林医药学院,基础部病原教研室,吉林,132013 2. 吉林医药学院,实验中心,吉林,132013 3. 吉林医药学院,科研处,吉林,132013 4. 吉林医药学院附属医院,泌尿外科,吉林,132011 |
| 摘 要： | 目的观察针对CD147RNA干扰对前列腺癌PC-3细胞生长的影响。方法用针对CD147分子的3个RNA干扰片段构建的表达载体转染PC-3细胞,获得稳定表达株。通过RT-PCR检测转染后CD147分子mRNA的表达水平变化;MTT法检测各组PC-3细胞增殖变化。结果RT-PCR结果发现第1、3干扰片段可以较好的封闭前列腺癌PC-3细胞CD147分子mRNA表达水平;MTT法显示CD147分子封闭后对前列腺癌PC-3细胞的生长无影响(P>0.05)。结论封闭CD147分子对前列腺癌PC-3细胞的生长无影响。 |
| 关 键 词： | RNA干扰 前列腺癌 生长 |
| 文章编号： | 1005-8982（2007）14-1720-03 |
| 收稿时间： | 2006-12-18 |
| 修稿时间： | 2006年12月18 |
CD147 RNA interference affect the growth of prostate cancer PC-3 cell line |
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| FANG Fang,FANG Qing,JU Xiao-hong,ZHONG Yue,WANG Li-guo.CD147 RNA interference affect the growth of prostate cancer PC-3 cell line[J].China Journal of Modern Medicine,2007,17(14):1720-1722. | |
| Authors: | FANG Fang FANG Qing JU Xiao-hong ZHONG Yue WANG Li-guo |
| Institution: | 1.Department of Pathogen, Jilin Medical College, Jilin 132013, P.R.China; 2.Department of Laboratory Center, Jilin Medical College, Jilin 132013, P.R.China; 3.Department of Scientific Research, Jilin Medical College, Jilin 132013, P.R.China;4.Department of Uroogy, the Affliatedcd Hospital of Jilin Medical College, Jilin 132013, P.R.China |
| Abstract: | Objective] To observe the effect of targeting CD147 RNA interference for the growth of prostate cancer PC-3 cell line.Methods] PC-3 cells were transfected with a vector coding for three pairs of siRNA targeting CD147.We obtained the stably expression PC-3 cell lines.RT-PCR was employed to determine the effect on expression of CD147 mRNA.The growth of PC-3 cell lines were detected through MTT.Results] Our results showed that CD147 expression was significantly inhibited by small interfering RNAs(siRNA)transfectants in PC-3 cells at mRNA level.MTT showed that there was no obvious difference in the RNAi transfected cells in growth(P >0.05).Conclusion] Targeting CD147 RNA interference didn't effect the growth of PC-3 cell line. |
| Keywords: | CD147 |
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