EGFr在原发性及交链孢酚诱导的食管癌中的扩增

用ＡＯＨ体外诱导不同时间（２４小时，１周和３周）的人胎儿食管上皮组织，提取该组织高分子量的ＤＮＡ，从１０例食管癌组织及癌旁“正常”组织中分别提以ＤＮＡ，用ＥｃｏＲＩ酶切，Ｓｏｕｔｈｅｒｎ转移，与ＥＧＦｒ基因探针杂交，结果显示：ＡＯＨ诱导２４小时人胎儿食管上皮中即有ＥＧＦｒ基因扩增，并一直持续到第三周，２例食管癌组织，１例癌旁“正常”组织也有ＥＧＦｒ基因扩增，因此：ＡＯＨ可以激活ＥＧＦｒ。ＥＧＦｒ基

THE AMPLIFICATION OF EGFr ONCOGENE IN PRIMARY ESOPHAGEAL CANCER AND IN AOH-INDUCED ESOPHAGEAL CANCER

High-molecular-weight DNA from the human fetal esophagusl (HFE) induced by alternariol(AOH) in vitro at different times(24 hours, 1 week and 3 weeks)in vitro was purified. Meanwhile it was isolated from 10 tumours and non-tumour adjacent tissues of the esophagus obtained from the patients who have undergone surgery for esophageal cancer. These DNA samples were also digested with EcoRI enzyme and subjected to southern blotting. The southetn blots was probed with EGFr gene. The result showed that EGFr gene in HFE induced by AOH in 24 hours has amplified and it couti- nued to the third week.The amplification of EGFr also existed in the two cases of esopha- geal cancer tissues and one case of non-tumour adjacent tissues of the esophagus, There- fore, EGFr can be activated by AOH. The activation of EGFr gene is probably due to the initiating change of the esophageal cancer and it played an important part in the genesis and deyelopment of the esophageal cancer.The amplification of EGFr in the non- tumour adjacent tissue should go beyond the present range of pathological diagnosis