间期荧光原位杂交技术检测慢性淋巴细胞白血病13q14缺失

目的探讨慢性淋巴细胞白血病（CLL）中13q14缺失[del（13q14）]情况。方法运用Spectrum Orange^TM标记的位于13q14的序列特异性DNA探针D13S319和间期荧光原位杂交（I—FISH）技术对83例初发的B细胞CLL（B-CLL）患者的细胞进行染色体13q14的检测。结果83例B-CLL中35例（42．2％）有del（13q14），其阳性细胞率为22．0％～95．0%，其中6例（7．2％）纯合缺失。del（13q14）在不同Binet分期中分别为A期27／63（42．9％）、B期7／15（46．7％）、C期1／5（20．ooA），各期之间无显著性差异（P〉0．05）。结论I-FISH是一种在分析CLL患者del（13q14）异常方面较为快速、准确和敏感的方法。del（13q14）在判断B-CLL预后方面的意义有待进一步探讨。

Detection of 13q14 deletion in chronic lymphocytic leukemia by interphase fluorescence in situ hybridization

Objective To investigate the incidence of 13q14 deletion del(13q14) in chronic lymphocytic leukemia(CLL).Methods Spectrum Orange~ TM labeled sequence-specific DNA probe D13S3 19 for 13q14 and interphase fluorescence in situ hybridization (I-FISH) were applied to detect del(13q14) in 83 patients with B-CLL.Results Of 83 patients,35(42.2%) had del(13q14) with the cell positive rate of 22.0% to 95.0%,and 6(7.2%) showed homogenous deletion.The incidences of del(13q14) in Binet stage A,B and C were 27/63(42.9%),7/15(46.7%) and 1/5(20.0%), respectively, which were not significantly different among three Binet stages(P>0.05).Conclusion I-FISH is a rapid,accurate and sensitive technique in analysing del(13q14) in CLL.The significance of del(13q14) in predicting the prognosis of B-CLL needs to be investigated further.