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| 口腔癌中人乳头瘤病毒16型和Ha-ras癌基因点突变的研究 | |
| 引用本文： | 周刚,程鹏,丁晓华,李辉热.口腔癌中人乳头瘤病毒16型和Ha-ras癌基因点突变的研究[J].临床口腔医学杂志,1999,15(4):214-215. |
| 作者姓名： | 周刚 程鹏 丁晓华 李辉热 |
| 作者单位： | 1. 430070,武汉,湖北医科大学口腔医学院 2. 北京医科大学口腔医学院 3. 湖北医科大学病毒研究所 |
| 摘 要： | 目的 检验口腔癌中的人乳头瘤病毒（ＨＰＶ）１６型ＤＮＡ和Ｈａ－ｒａｒ癌基因的点突变，探讨ＨＰＶ１６型和Ｈａ－ｒａｓ癌基因的点突变与口腔癌之间的关系。方法 应用多聚酶链反应（ＰＣＲ）技术扩增ＨＰＶ１６ＤＮＡ和Ｈａ－ｒａｓ癌基因相关片段，分别采用２％琼脂糖凝胶电泳和限制性片段长度多态性分析（ＲＦＬＰ）检测口腔癌中的ＨＰＶ１６ＤＮＡ和Ｈａ－ｒａｓ癌基因第１２位密码子的点突变，结果 在１７例口腔癌组中，６ |
| 关 键 词： | 口腔癌 人乳头瘤病毒16型 Ha-ras癌基因 点突变 |
Human Papillomavirus 16 and point mutation of Ha-ras oncogene in oral squamous cell carcinoma |
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| zhou Gang,Cheng Peng,Ding Xiaohua,et al..Human Papillomavirus 16 and point mutation of Ha-ras oncogene in oral squamous cell carcinoma[J].Journal of Clinical Stomatology,1999,15(4):214-215. | |
| Authors: | zhou Gang Cheng Peng Ding Xiaohua |
| Institution: | zhou Gang,Cheng Peng,Ding Xiaohua,et al. College of Stomatology,Hubei Medical University,Wuhan 430070 |
| Abstract: | Objective To study the association of Human papillomavirus (HPV) 16 and point mutation of Ha-ras oncogene with oral squamous cell carcinoma. Methods Polymerase chain reaction(PCR) was used to detect HPV 16 DNA and point mutation at condon 12 of Ha-ras oncogene. Results 6 of 17(35.3%) cases of oral squamous cell carcinoma were proved to contain HPV16 DNA. 5(29.4%)were found to have point mutation of Ha-ras oncogene. Conclusion HPV16 and point mutation of Ha-ras oncogene might play some role in the carcinogenesis of oral squamous cell carcinona. |
| Keywords: | Oral squamou cell carcinoma Human papillomavirus 16 Ha-ras oncogene Point mutation |
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