| 高效液相色谱法测定孕妇血清中色氨酸及其代谢产物Kynurenine |
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| 引用本文： | 徐雪梅,夏晓萍.高效液相色谱法测定孕妇血清中色氨酸及其代谢产物Kynurenine[J].浙江中医药大学学报,2007,31(2):151-152. |
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| 作者姓名： | 徐雪梅 夏晓萍 |
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| 作者单位： | 1. 浙江中医药大学附属第一医院,杭州,310006
2. 浙江大学医学院附属妇产科医院 |
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| 摘 要： | 目的]建立一种高效液相色谱法测定孕妇血清中的色氨酸（Tyrptophan）及其代谢产物Kynurenine的方法。方法]反相高效液相色谱法。外标法，ZORBAX Extend-C18柱，4．6mmID×250mm，流动相为甲醇：0．001mol／L磷酸二氢钾（采用PH=4）（12：88），流速1．3ml／min，检测波长245nm。结果]色氨酸线性范围2．56～307．06μmol／L，r=0．9999；Kynurenine线性范围0．12～5．05μmol／L，r=0．9997。30例孕妇血清色氨酸为49．73±7．48μmol／L，其代谢产物KN为：0．74±0．33μmol／L。结论]本法简便，准确，适用于临床和科研。
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| 关 键 词： | 高效液相色谱法 色氨酸(Tyrptophan) Kynurenine孕妇测定 |
| 文章编号： | 1005-5509（2007）02-0150-02 |
| 修稿时间： | 2006-03-02 |
Determination of Serum Tryptophan and Kynurenine in Pregnant Women |
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| Xu Xuemei,Xia Xiaoping.Determination of Serum Tryptophan and Kynurenine in Pregnant Women[J].Journal of Zhejiang University of Traditional Chinese Medicine,2007,31(2):151-152. |
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| Authors: | Xu Xuemei Xia Xiaoping |
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| Abstract: | Objective] To establish a method for determination of serum tryptophan and kynurenine in pregnant women. Method] RP HPLC was applied to analysis. C18 column was used. The mobile phase was composed of methanol-0, 001M KH2PO4（PH=4） （12 ： 88）. The UV detection wave length was 245nm. Results] The linear response ranged from 2.56-307.06μmol·L^-1 for tryptophan, r=0. 9999. The linear response ranged from 0. 12-5.05μmol·L^-1 for kynurenine, r=0. 9997. The mean concentrations of serum tryptophan and kynurenine were 49.73±7.48μmol·L^-1 and 0. 74±0. 33μmol·L^-1 respectively in 30 pregnant women. Conclusion] The method is sensitive and suitable for the determination. |
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| Keywords: | Kynurenine |
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