岗梅根总皂苷对高脂血症大鼠降血脂作用的实验研究

目的:探讨岗梅根总皂苷对高血脂模型大鼠的降血脂作用。方法:将72只大鼠,随机分为6组,正常组、模型组、辛伐他汀组、岗梅总皂苷高、中、低剂量组。通过给予大鼠饲喂高脂饲料,建立高血脂大鼠模型。正常组和模型组均灌胃等体积生理盐水,辛伐他汀组灌胃辛伐他汀4 mg/kg,岗梅总皂苷高、中、低剂量组分别灌胃440 mg/kg、220 mg/kg、110 mg/g岗梅根总皂苷混悬液。连续给药10周,每天1次。测量血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血清瘦素(LEP)、脂联素(ADP)、白细胞介素-6(IL-6)、C-反应蛋白(CPR)水平。结果:与模型组比较,岗梅根总皂苷高、中、低剂量组大鼠血清中TC、TG、LDL-C、LEP、CRP和IL-6水平均显著降低(P0.01),岗梅根总皂苷各剂量组HDL-C水平均显著升高(P0.01,P0.05),岗梅根总皂苷高剂量可显著升高ADP水平(P0.05)。结论:岗梅根总皂苷对高血脂大鼠有降血脂作用。

Experiment Research of Total Saponins from Radix Ilicis Asprellae for Decreasing Blood Lipid in Hyperlipidemia Rats

Objective. To observe the effect of total saponins from Radix Ilicis Asprellae for decreasing blood lipid in hyperlipidemia rats. Methods： Seventy two rats were randomly divided into six groups, namely normal group, model group, Simvastetin group, and high （H）-, middle（M）-, Iow（L）-dose Radix Ilicis Asprellae groups. After the establishment of hyperlipidemia model by high-fat feeding, the normal group and the model group were given normal saline with the same volume as that for other groups, and Simvastetin group was administrated with Simvastetin, 4mg for per kilogram of body weight, while H group, M group and L group were administrated with total saponins from Radix Ilicis Asprellae 440 mg, 220 mg, 110 mg for per kilogram of body weight respectively. The treatment course lasted 10 weeks. The levels of serum total cholesterol（TC）, triglyceride（TG）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, leptin（LEP）, adiponcetin（ADP）, interleukin-6（IL-6） and C-reactive protein （CRP） were observed. Results= The levels of TC, TG, LDL-C, LEP, CRP and IL-6 were decreased（P 〈 0.01） and HDL-C was increased（P 〈 0.05） in H, M, L groups, and ADP was increased （P 〈 0.05） in H group after treatment as compared with those in the model group. Conclusion： Total saponins from Radix Ilicis Asprellae can markedly decrease blood lipid in hyperlipidemia rats.