补肾健骨方含药血清对ROBs和rBMSCs增殖、分化和矿化的影响

目的 观察补肾健骨方含药血清对大鼠颅骨成骨细胞(rat calvarial osteoblasts,ROBs)和大鼠骨髓间充质干细胞(rat bone marrow mesenchymal stem cells,rBMSCs)细胞活性及分化、矿化的影响.方法 制备补肾健骨方含药血清,将ROBs和rBMSCs分为5％、1...

Effect of nourishing kidney and strong bone recipe-containing serum on the proliferation and differentiation of osteoblasts and bone marrow mesenchymal stem cells in rats

Objective To observe the effect of nourishing kidney and strong bone recipe-containing serum on the proliferation and differentiation of rat calvarial osteoblasts (ROBs) and bone marrow mesenchymal stem cells (rBMSCs). Methods The nourishing kidney and strong bone recipe-containing serum was prepared. ROBs and rBMSCs were divided into blank serum group, 5%, 10%, 15%, 20%, and 25% concentration drug-containing serum group, and traditional induction group. MTT method was used to detect the proliferation of ROBs and rBMSCs. Alkaline phosphatase (ALP) kit was used to detect the ALP activity of ROBs and rBMSCs. Alizarin red staining was used to detect the formation of mineralized nodules. Results Compared with those in the blank serum group and the traditional induction group, the drug-containing serum groups at different concentrations promoted the proliferation of ROBs and rBMSCs to different degrees, increased the ALP activity, and increased the number and area of calcified nodules (P<0.05). Among them, the effect of promoting the proliferation and differentiation of ROBs was the best at the concentration of 20% of drug-containing serum. The effect of promoting the proliferation and differentiation of rBMSCs was the best at the concentration of 10% of drug-containing serum. Conclusion The nourishing kidney and strong bone recipe-containing serum promotes the osteogenic proliferation and differentiation of ROBs and rBMSCs at the concentration of 20% and 10%., respectively.