| 姜黄素对同型半胱氨酸硫内酯所致Eahy926细胞损伤的保护作用 |
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| 引用本文： | 刘玉晖,彭杏,杨丹,袁禄根. 姜黄素对同型半胱氨酸硫内酯所致Eahy926细胞损伤的保护作用[J]. 时珍国医国药, 2012, 23(8): 1898-1901 |
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| 作者姓名： | 刘玉晖 彭杏 杨丹 袁禄根 |
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| 作者单位： | 江西中医学院·药学院,江西南昌330004 |
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| 基金项目： | 江西省自然科学基金(No.2008GQY001);江西省教育厅青年科学基金项目(No.GJJ09545) |
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| 摘 要： | 目的 研究姜黄素对同型半胱氨酸硫内酯(HTL)诱导融合内皮细胞株(eahy926)损伤的保护作用及其机制.方法 以体外培养的eahy926细胞为研究对象,建立HTL损伤模型,检测LDH漏出量,SOD和MDA含量,通过RT-PCR法检测细胞中对氧磷酶1( PONl) mRNA,TNF-α mRNA水平.结果 姜黄素可明显降低培养液中LDH活力,显著提高培养液中SOD含量,降低MDA含量；抑制HTL刺激的培养液中TNF-α浓度的升高,提高细胞中PONl mRNA水平,降低TNF-αmRNA表达.结论姜黄素对HTL诱导的eahy926细胞损伤具有一定保护作用.
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| 关 键 词： | 姜黄素 内皮细胞 同型半胱氨酸硫内酯 对氧磷酶1 |
Protective Effects of Curcumin against Injury of Eahy926 Cells Induced by Homocysteine Thiolactone |
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| LIU Yu-hui , PENG Xing , YANG Dan , YUAN Lu-gen. Protective Effects of Curcumin against Injury of Eahy926 Cells Induced by Homocysteine Thiolactone[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(8): 1898-1901 |
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| Authors: | LIU Yu-hui PENG Xing YANG Dan YUAN Lu-gen |
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| Affiliation: | (College of Pharmacology,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China) |
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| Abstract: | Objective To observe the mechanism and protective effects of Curcumin against injury of Eahy 926 Cells induced by homocysteine thiolactone(HTL) MethodsEahy 926 cells were cultured with HTL as the object of study.The contents of lactate dehydrogenase(LDH),superoxide dismutase(SOD),malondialdehyde(MDA) in medium were detected.The expression of PON1mRNA,TNF-αmRNA cell were estimated by RT-PCR.Results Pretreatment with curcumin obviously increased the activity of cell SOD,decreased the levels of LDH,MDA,TNF-α,up-regulated the PON1mRNA and down-regulated the TNF-αmRNA.Conclusion Curcumin has protective effects against injury of eahy926 cells induced by HTL. |
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| Keywords: | Curcumin eahy926 HTL PON1 |
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