脂联素基因多态性和血清脂联素水平与非酒精性脂肪肝的相关性

目的:探讨脂联素基因单核苷酸多态性及血清脂联素水平与汉族人非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)的关系.方法:根据性别、年龄和体质量指数(body-mass index,BMI)配对入选NAFLD患者和对照组,各106例.测定基因多态性和血清脂联素水平,分析其与人体测量参数、生化、激素和代谢的关系.结果:在NAFLD和对照者中,SNP+45位点T/T、T/G和G/G基因型分别为64.2%、54.7%和23.6%、38.7%和12.2%、6.6%,NAFLD组G/G基因型频率比对照组高(2=6.47,P<0.05),但两组等位基因型频率(2=0.64,P>0.05)差异无显著性;S N P+276位点中G/G、T/G和T/T基因型分别为4.2%、47.2%和27.3%、36.8%和8.5%、16.0%,NAFLD组T/T基因型频率高于对照组(2=6.68,P<0.05),两组等位基因频率差异有显著性(2=7.86,P<0.05).NAFLD组的血清脂联素水平较对照组显著降低(3.75mg/L±3.94mg/Lvs6.18mg/L±4.12mg/L),而且有更高的胰岛素低抗(2.19±1.35vs1.33±0.93).Logistic回归分析显示:BMI、WHR、HOMA-IR、脂联素是NAFLD的主要危险因素.结论:SNP45G/G和SNP276T/T基因型可能是中国汉族人NAFLD的易感基因,高BMI、WHR、HOMA-IR、低脂联素血症是NAFLD的主要危险因素.

Association of single nucleotide polymorphisms of the adiponectin gene and plasma levels of adiponectin with risk of nonalcoholic fatty liver disease

AIM:To investigate the relationship among the adiponectin gene polymorphisms,plasma levels of adiponectin,and risk of nonalcoholic fatty liver disease(NAFLD) in Cantonese Han people.METHODS:We measured the adiponectin gene polymorphisms and plasma adiponectin levels in 106 NAFLD patients and 106 controls with comparable age,sex and body-mass index(BMI) and analyzed their correlation with anthropometric,biochemical,hormonal and metabolic parameters.RESULTS:The T/T,T/G and G/G genotypes of the SNP 45 locus were found in 64.2%,23.6% and 12.2% of NAFLD patients,and in 54.7%,38.7% and 16.6% of controls,respectively.The frequency of GG genotyp in NAFLD patients was higher than that in controls(2 = 6.47,P < 0.05).The allelic frequency between two groups showed no significant difference(2 = 0.20,P > 0.05).The G/G,T/G and T/T genotypes of the SNP 276 locus were found in 64.2%,27.3% and 8.5% of NAFLD patients,and in 47.2%,36.8% and 16% of controls,respectively.The frequency of T/T genotype in NAFLD patients was higher than that in controls(2 = 6.68,P < 0.05).There was a significant difference in the allelic frequency between the two groups(2 = 7.86,P < 0.05).Plasma adiponectin levels were significantly lower in NAFLD patients than in controls(3.75 mg/L ± 3.94 mg/L vs 6.18 mg/L ± 4.12 mg/L,P < 0.05).Moreover,NAFLD patients had more significant insulin resistance(2.19 ± 1.35 vs 1.33 ± 0.93,P < 0.05).Logistic regression analysis showed that BMI,waist to hip ratio(WHR),HOMA-IR,adiponectin were main risk factors for NAFLD.CONCLUSION:The SNP 45G/G and SNP 276T/T genotypes of the adiponectin gene are associated with risk of NAFLD.High BMI,high WHR,elevated HOMA-IR,and hypoadiponectin are main risk factors for NAFLD.