阿里红中总三萜酸的提取工艺优选及含量测定

目的:优化阿里红中总三萜酸的提取工艺,并建立其含量测定方法。方法:以齐墩果酸为对照品,采用分光光度法测定三萜酸含量;采用单因素试验考察提取溶剂种类及浓度、料液比、温度、提取时间及提取次数对总三萜酸得率的影响,在此基础上,采用L9(34)正交试验优选阿里红中总三萜酸提取工艺。结果:最佳提取工艺为30倍量90%乙醇于60℃超声提取3次,每次30 min。在此条件下,阿里红中总三萜酸质量分数35.1%。各因素影响次序为提取温度>液料比>提取时间>乙醇体积分数。结论:阿里红中三萜酸含量很高,该优选工艺稳定可行,可推广于大生产。

Optimization of Ultrasonic Extraction Technology and Content Determination of Total Tritepenoidic Acid from Fomes officinalis

Objective:To optimize extraction technology of total tritepenoidic acid from Fomes officinalis and establish its content determination method.Method:The content of total tritepenoidic acid was determined by UV-Vis with oleanic acid as reference;Effects of kind and concentration of extraction solvent,solid-liquid ratio,temperature,extraction time and extraction times on yield of total tritepenoidic acid were investigated by single factor test,basis on this,extraction technology was optimized by orthogonal test.Result:Optimum conditions of ultrasonics extraction were as follow:extracted 3 times at 60 ℃ with 30 times the amount of 90% ethanol,30 min each time.Under these conditions,effect of factors on yield was in order of extraction temperature>solid-liquid ratio>extraction time>the concentration of ethanol,yield of total tritepenoidic acid from F.Officinalis was 35.1%.Conclusion:The content of triterpenoidic acid from F.officinalis was very high,this optimized technology was stable and feasible,which could be extended to large-scale production.