How do patch clamp seals form? A lipid bleb model |
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| Authors: | R. L. Milton J. H. Caldwell |
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| Affiliation: | (1) Departments of Cellular and Structural Biology, University of Colorado Health Sciences Center, 80262 Denver, CO, USA;(2) Department of Physiology, University of Colorado Health Sciences Center, 80262 Denver, CO, USA;(3) Present address: Indiana University School of Medicine, Muncie Center for Medical Education, Ball State University, 47306 Muncie, IN, USA |
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| Abstract: | Individual ion channels are electrically isolated and studied in living cells with the tight patch voltage clamp method. Channels are identified, categorized, and sometimes named on the basis of the biophysical properties obtained with this method. Although it is usually presumed that these recordings are from native, undisturbed membrane, the physical basis of this technique is not well established. Observations that lipid blebs readily form when suction is applied to patch clamp electrodes suggest that many single channel recordings are from ion channels in these blebs. |
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| Keywords: | Patch clamp technique Membrane blebs Skeletal muscle |
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