骨形态发生蛋白-7在前列腺癌组织中的表达及意义

目的 研究骨形态发生蛋白-7(BMP-7)在前列腺癌(PCa)组织中的表达及意义.方法 经病理检查确诊PCa组织标本87例.患者平均年龄66(59～78)岁,术前或穿刺前血清总PSA(t-PSA)平均45.7(2.4～138.2)ng/ml.Gleason评分≤6分37例,7分18例,≥8分32例.临床分期:I期(T1a N0 M0)+Ⅱ期(T1b N0M0,T1cN0M0,T2N0M0)20例,Ⅲ期(T3N0M0)20例.Ⅳ期(T4N0M0,TxN1M0,TxN0M1)47例,其中TxN0M1 45例.根据核素骨扫描或正电子发射计算机体层成像CT检查结果分为:PCa无骨转移42例,PCa伴骨转移45例.以30例BPH组织标本为对照,患者平均年龄68(57～88)岁.免疫组织化学PV法检测BMP-7在PCa组织和BPH组织中的表达,统计学分析2组及PCa组内表达差异,PCa组织中BMP-7与血清t-PSA相关性采用Pearson相关性分析. 结果BPH组织中BMP-7表达吸光度A值为70.55±5.41,PCa组织中为70.47±6.18,2者比较差异无统计学意义(P>0.05).PCa无骨转移组BMP-7表达吸光度A值为65.94±1.76,伴有骨转移组为74.80±5.76,2者比较差异有统计学意义(P<0.05).Gleason评分≤6分者吸光度A值为65.96±1.56,7分者为65.83±2.75,≥8分者为78.06±1.39.≤6分和7分者分别与≥8分相比,BMP-7表达A值差异有统计学意义(P<0.05).临床分期编组中Ⅰ期+Ⅱ期BMP-7表达A值为65.86±1.72,Ⅲ期为65.87±1.85,Ⅳ期为74.49±5.83,Ⅰ期+Ⅱ期和Ⅲ期分别与Ⅳ期相比,差异有统计学意义(P<0.05).PCa组织中BMP-7表达A值与血清t-PSA值呈正相关关系(r=0.77,P<0.05).结论 病理Gleason高评分、临床分期、已发生骨转移的PCa组织中高表达BMP-7,BMP-7表达水平与血清t-PSA具有正相关性,提示BMP-7可能是促进PCa细胞发生骨转移的重要细胞因子之一.

Expression of bone morphogenetic protein-7 in prostate cancer

Objective To investigate the expression of bone morphogenetic protein-7(BMP-7)in the tissue of prostate cancer(PCa). Methods The pathological samples of 87 cases of PCa were collected.The average age was 66(59-78)years,preoperative of t-PSA was 45.7(2.4-138.2)ng/ml.Gleason score:37 cases were≤6,18 cases were 7,32 cases were≥8.Stages:stage I(T1aN0M0)+stageⅡ(T1bN0M0,T1cN0M0,T2N0M0)20 cases;StageⅢ(T3N0M0)20;Stage Ⅳ(T4N0 M0,TxN1 M0,TxN0 M1)47 cases.According to bone scan or positron emission computed tomography-CT test results,patients were divided into PCa without bone metastasis,42 cases and PCa with bone metastasis,45 cases.Thirty cases of BPH were set as controls.BMP-7 in the PCa and BPH were detected by PV immunohistochemical study.Statistical analysis was done between two groups to compare the differential expression of BMP-7 and serum t-PSA in PCa, and BPH tissues.Results BMP-7 expression in the absorbance A value in benign prostatic hyperplasia was 70.55±5.41, in prostate cancer tissue 70.47± 6.18, no significant differences between the 2 groups(P>0.05).BMP-7 expression in the absorbance A value in prostate cancer without bone metastasis was 65.94 ± 1.76, but with bone metastasis 74.80±5.76.There was a significant difference (P<0.05).Gleason score≤6 absorbance A value was 65.96 ± 1.56, Gleason 7 absorbance A value 65.83 ± 2.75,≥8 absorbance A value 78.06±1.39.Compared with Gleason score≥8, BMP-7 expression in the absorbance A value were significantly lower than the latter (P<0.05).Clinical stage grouping of BMP-7 expression in the absorbance A value: Stage Ⅰ + Ⅱ 65.86±1.72, Stage Ⅲ 65.87±1.85, Stage 74.49±5.83.There was a significant difference (P<0.05).In PCa tissues, BMP-7 of the absorbance A value and the serum t-PSA values showed a positive correlation (r=0.77,P,<0.05). Conelusions The expression level of BMP-7 has occurred in the high pathological Gleason score, late clinical stage, particularly in bone metastasis cases.The expression level of BMP-7 and serum t-PSA have a positive correlation.