内皮抑素抑制黑色素瘤浸润转移的实验研究

【目的】研究内皮抑素对小鼠B16黑色素瘤中基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）及组织基质金属蛋白酶抑制剂-2（TMP-2）表达的影响，并阐述相关分子机制。【方法】建立小鼠B16黑色素瘤动物模型，分为内皮抑素实验组和空白对照组，构建组织芯片，应用免疫组化方法检测两组肿瘤组织MMP-9、MMP-2及TIMP-2的表达。【结果】MMP-2、MMP-9和TIMP-2在实验组与对照组间的表达差异均具有统计学意义（t=15．002，t=9．852，t=12．893，P〈0．05）。【结论】内皮抑素影响黑色素瘤组织表达MMP-2和MMP-9，抑制肿瘤生长和局部浸润转移。

Experimental study on the inhibition of infiltration and metastasis of melanoma by endostatin

Objective] To investigate the effect of endostatin on the expression of MMP-2, MMP-9 and their inhibitor TIMP-2 in mice B16 melanoma tissue and to explore the relevant molecular mechanism. Methods] Establishing B16 melanoma mice transplantation tumor model. Dividing the mice into experimental group and control group. Treating the mice in experimental group with endastatin, while the control group with no treatment. The expression of matrix metalloproteinase-2, -9 （MMP-2, MMP-9） and tissue inhibitor of matrix metaloproteinase-2 （TIMP-2） were detected by tissue array and immunohistochemieal staining. Results] The expression of MMP-2, MMP-9 and TIMP-2 in treated group were all statistically different as compared with the control group. （ t = 15.002, t = 9.852 , t = 12.893 , P 〈 0.05 ） Conclusions] Endostatin influences the expression of MMP-2 and MMP- 9 in melamoma, inhibiting tumor growth and metastasis.