| 转基因牛乳中重组人乳铁蛋白的仿生亲和纯化 |
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| 引用本文： | 文胜,田麟,肖天舒,冉启迪,董德贤,李荣秀.转基因牛乳中重组人乳铁蛋白的仿生亲和纯化[J].中国生化药物杂志,2009,30(2). |
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| 作者姓名： | 文胜 田麟 肖天舒 冉启迪 董德贤 李荣秀 |
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| 作者单位： | 上海交通大学,生命科学技术学院,上海200240 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展规划(973计划) |
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| 摘 要： | 目的从转基因牛乳中亲和纯化重组人乳铁蛋白。方法从仿生亲和介质库中筛选亲和配体,进行重组人乳铁蛋白的纯化研究。结果筛选出可以特异性结合重组人乳铁蛋白的固定化β-丙氨酸亲和配体,从转基因牛乳中一步纯化的重组人乳铁蛋白纯度为95.2%,蛋白回收率可达81.9%。经放大实验表明该亲和纯化工艺适合工业上大规模纯化乳铁蛋白。结论固定化β-丙氨酸可从转基因牛乳中纯化出高纯度重组人乳铁蛋白,工艺适合工业化生产。
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| 关 键 词： | 重组人乳铁蛋白 仿生亲和配体 亲和纯化 |
Biomimetic affinity purification of recombinant human lactoferrin from the milk of transgenic cows |
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| WEN Sheng,TIAN Lin,XIAO Tian-shu,RAN Qi-di,DONG De-xian,LI Rong-xiu.Biomimetic affinity purification of recombinant human lactoferrin from the milk of transgenic cows[J].Chinese Journal of Biochemical Pharmaceutics,2009,30(2). |
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| Authors: | WEN Sheng TIAN Lin XIAO Tian-shu RAN Qi-di DONG De-xian LI Rong-xiu |
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| Institution: | School of Life Science and Biotechnology;Shanghai Jiao Tong University;Shanghai 200240;China |
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| Abstract: | Purpose To purify the recombinant human lactoferrin from the milk of transgenic cows by affinity chromatography.Methods A library of biomimetic affinity ligands was screened for lactoferrin binding specificity and capacity.Affinity chromatography was carried out based on the synthetic ligand selected.Results Immobilised β-Alanine was found to be an affinity ligand specific for recombinant human lactoferrin.By this ligand,recombinant human lactoferrin was one-step purified from the milk of transgenic cows with a purity of 95.2% and the protein recovery could reach 81.9%.Conclusion Immobilised β-Alanine can be used to purify recombinant human lactoferrin,and this process was scalable and suitable for commercialization. |
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| Keywords: | recombinant human lactoferrin biomimetic affinity ligand affinity purification |
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