鳀鱼提取物(鱼酱油)对大鼠血糖及体重影响的实验研究

目的 研究鱼酱油对实验性糖尿病动物是否具有降血糖作用。方法 用体重为200—220g的SD大鼠200只，禁食24h，尾静脉注射四氧嘧啶60mg／kg，建立高血糖模型。观察高剂量（5ml／kg）、中剂量（2.5ml／kg）和低剂量（1．25ml／kg）鱼酱油对实验性糖尿病大鼠空腹血糖和糖耐量的影响。用正常SD大鼠观察高剂量鱼酱油对正常大鼠的体重及血清Cr，BUN，GPT，GOT等指标的影响。结果 连续给予高、中、低剂量鱼酱油30d，对高血糖大鼠空腹血糖均无明显影响（P＞0．05），但可引起糖尿病大鼠糖耐量试验的血糖曲线下面积明显下降（P〈0．01或〈0．05）；连续给予高剂量鱼酱油45d，引起正常大鼠体重增长减慢（P〈0．05）。结论 高、中、低剂量鱼酱油均可降低高血糖大鼠糖耐量试验中的血糖曲线下面积，即提高受试动物的血糖耐受水平；高剂量（5ml／kg）鱼酱油可减慢正常大鼠的体重增长。

Experimental research on the effects of Engraulis japonicus extractum ( fish sauce) on blood glucose and weight of rats

Objective To investigate the effect of Engraulis japonicus extractum (fish sauce) in reducing blood glucose level and weight of experimental diabetes rats. Methods Two hundred SD rats weighed from 200 g to 220 g were fasted for 24 hours,then were injected alloxan in tail-vein with the dose of 60 mg/kg to build as experimental diabetes animal models. Effects of different doses of Engraulis japonicus extractum (fish sauce) (high dose of5 ml/kg, middle dose of 2. 5 ml/kg, low dose of 1.25 ml/kg) on fasting blood glucose and gluscose tolerance of these models were observed. And effects of high dose of Engraulis japonicus extractum (fish sauce) on weight,serum Cr,BUN,GPT,GOT of normal SD rats were examined. Results Given high, middle, low dose of Engraulis japonicus extractum (fish sauce) constantly for 30 days, no significant change in blood glucose was observed in the models (P > 0. 05) , but the area below blood glucose curve in glucose tolerance test figure was evidently reduced (P < 0. 01 or < 0. 05) . Given high dose of Engraulis japonicus extractum (fish sauce) to normal SD rats constantly for 45 days, weight-increasing speed of the rats was significant reduced (P < 0.05). Conclusions Taking high, middle, low dose of Engraulis japonicus extractum (fish sauce) can help to reduce the area below blood glucose curve in glucose tolerance test figure, in other words, to increase glucose tolerance threshold of diabetes rats. Taking high dose of Engraulis japonicus extractum (fish sauce) (5 ml/kg) can help to decrease the weight-increasing speed of normal rats.