miR-224和 miR-378e在大肠癌组织中的表达及其临床意义

【摘要】 目的 探讨miR-224 和 miR-378e 在原位大肠癌癌组织和癌旁正常黏膜组织中的表达差异， 并分析其临床意义。 方法 miRNA 表达谱芯片技术检测大肠癌癌组织和癌旁组织标本中表达差异的miRNA， 运用荧光实时定量聚合酶链反应（real-time PCR）验证其结果，并分析其与临床病理资料之间的关系。 结果 miRNA 表达芯片分析发现， 与正常黏膜组织比较， miR-224在大肠癌组织中表达显著上调， miR-378e在大肠癌组织中表达显著下调， 并被 real-time PCR 所证实。 miR-224 在不同组织学类型癌组织中表达差异有统计学意义， miR-378e 在不同浸润深度癌组织中表达差异有统计学意义， miR-224 表达与组织学类型有关， miR-378e 表达与大肠癌浸润深度有关。 结论miR-224具有潜在的癌基因作用， miR-378e具有潜在的抑癌基因作用， miR-224和 miR-378e可作为

Expression and Clinical Significance of miR-224 and miR-378e in Colorectal Cancer Tissues

Objective To investigate the expression and clinical significance of microRNA-224 and microRNA -378e in non-metastatic colonrectal cancer tissues and non-cancerous tissues surrounding colonrectal cancer in situ .Methods The expression level of miR-224 and miR-378e in tumor and adjacent normal tissue from clinical colonrectal cancer patients without metastasis was detected by Chip - array . And the determination results were verified by real - time PCR . The correlation of miR-224 and miR-378e expession and clinicopathological features of colonrectal cancer was analyzed . Results The expression level of miR-224 was significantly up-regulated in tumor tissue (P<0 .001)while miR-378e was down-regulated in tumor tissue , and validated by real-time PCR . The expression of miR-224 was strongly associated with Histological type(P<0 .05)，while miR-378e with Infiltration depth(P<0 .05) . Conclusion miR-224 is a potent tumor promoter , while miR-378e is a potent tumor suppressor .