茶多酚治疗N-甲基亚硝基脲诱发膀胱肿瘤的实验研究

【摘要】 目的 观察茶多酚对N-甲基亚硝基脲（MNU）诱发膀胱肿瘤的治疗作用。方法 将72只雌性Wistar大鼠随机分为治疗组和对照组，各36只。经膀胱内灌注MNU（2mL/次，2周1次）制作膀胱肿瘤模型，治疗组以茶多酚溶液灌胃，对照组以蒸馏水灌胃，每日1次，每次2mL。实验开始后第3、5、7、9、11和13周，每组各处死6只大鼠，取其膀胱组织标本，显微镜下观察2组膀胱组织病理变化情况并记分。结果 对照组从第5周开始，膀胱黏膜出现明显的不典型增生病变，第11周开始发生膀胱恶性肿瘤；治疗组始终未见膀胱恶性肿瘤。2组病理学变化记分除第3周差异不明显外（P＞0.05），其余时间段均是治疗组小于对照组（P＜0.05）。对照组大鼠膀胱壁黏膜第3周增厚至5~7层，第7周出现乳头状瘤样变化，第11周出现部分癌变，至第13周细胞异形性明显，排列紊乱，核分裂象明显。治疗组在第5~13周细胞层次轻度增厚至3~5层，细胞排列有序，核浆比例基本正常。结论 茶多酚可有效治疗MNU诱发的膀胱肿瘤。

Observation on Epigallocatechin Gallate Treating the Bladder Tumor Induced by N-methyl-N-nitrosourea in Rats

[Abstract] Objective Toinvestigate the therapeutic effect of epigallocatechin gallate (EGCG) on the bladder neo-plasm induced by N-methyl-N-nitrosourea (MNU) in Wistar rats.Methods Seventy-two female rats were randomly divided into two groups, treatment group and control group. The rat model of bladder tumor was established by intravesical instillation of MNU (2mL/time, once every two weeks) inside bladder. Rats in treatment group were given EGCG (2mL/time, once/day), and control group were given the same volume of distilled water. Six rats were executed respectively at the3^rd, 5^th,7^th,9^th, 11^th and13^th week, and the bladder histopathological changes were studied in two groups.ResultsThe untypical hyper-plasia was observed after5weeks. The bladder neoplasm was observed at the11^th week in control group. The bladder neo-plasm was not found in treatment group. There was a significantly lower pathological score in treatment group than that of control group except for the first two weeks (P＜0.05).Conclusion EGCG is effective for treatment of the bladder neo-plasm induced by MNU.