Protective immunity against<Emphasis Type="Italic"> Toxoplasma gondii</Emphasis> in mice induced by the SAG2 internal image of anti-idiotype antibody |
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Authors: | Chung-Dar?Yang Gan-Nan?Chang Email author" target="_blank">David?ChaoEmail author |
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Institution: | (1) Department of Biological Science, National Sun Yat-sen University, Kaohsiung, Taiwan;(2) Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan |
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Abstract: | A monoclonal antibody TGCM5 (isotype G, subclass 1, light chain) against SAG2, a major surface antigen of Toxoplasma gondii tachyzoites, was produced in quantity and its Fab-containing idiotype (Id) was injected into rabbits for production of rabbit anti-Id sera. The rabbit anti-Id IgG (aId-IgG) with a SAG2 internal image was obtained by purification with a protein A column. Significant lymphocyte proliferation induced by tachyzoite lysate antigen was observed in BALB/c mice that received two injections of aId-IgG, but not in non-immunized control mice. Large amounts of interferon were detected in supernatants of splenocyte cultures prepared from mice immunized with aId-IgG. Of the mice immunized with aId-IgG, 75–87.5% survived at least 28 days after a lethal challenge of 1×103 live tachyzoites, which killed all non-immunized control mice within 10 days. The results showed that the SAG2 internal image presented by aId-IgG indeed elicited a protection against the infection of T. gondii in mice. |
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