Survivin在大肠癌中的表达及其与血管生长因子的相关性研究

目的　检测大肠癌中凋亡抑制基因Survivin的表达,研究其与大肠癌临床特征和血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)表达的相关性,探讨Survivin在大肠癌发生、发展及转移中的作用及机制。方法　收集6 2例大肠癌标本及其病例资料,应用免疫组织化学方法检测Survivin、VEGF、bFGF在大肠癌标本中的表达情况。结果　Survivin、VEGF、bFGF基因在癌旁正常大肠组织中均不表达,在6 2例大肠癌组织中,30例Survivin表达阳性,阳性率为4 8. 39% ,Survivin与大肠癌的Ducks分期密切相关(P <0 .0 0 1)但与病理分化无关(P >0 .0 5 )。2 9例VEGF表达阳性,阳性率4 6 . 18% ,4 5例bFGF表达阳性,阳性率75 . 5 8%。肿瘤局限于黏膜层及黏膜下层和肌层、浆膜层时,VEGF、bFGF表达无明显差异(P >0 . 0 5 ) ,但当肿瘤侵犯到浆膜层和浆膜外时,VEGF、bFGF蛋白表达阳性率明显增高(P<0 . 0 0 1)。淋巴转移组的VEGF、bFGF阳性表达显著高于未发生淋巴转移组(P <0. 0 0 0 1)。结论　Survivin基因、VEGF、bFGF与大肠癌的Ducks分期有密切关系但与病理分化无关,检测Survivin更有意义。

Expression of Survivin, bFGF and VEGF and its relationship with colorectal carcinoma

Objective To investigate the expression of survivin, bFGF and VEGF in human colorectal carcinoma tissue and evaluate their value as a tumor marker and clinical prognostic indicator. Methods The protein expression of survivin, bFGF and VEGF were examined in 62 colorectal carcinoma by immunohistochemical method(SP technique). The relationship between the expression of survivin, bFGF and VEGF protein in human colorectal carcinoma and clinical pathologic parameters was analyzed. Results There were no expression of survivin, bFGF and VEGF protein in normal colorectal tissues. The positive rate of survivin, bFGF and VEGFi in 62 colorectal carcinoma respectively was 48.39%(30/62), 46.18%(29/62), 75.58%(45/62). The expression of survivin had a close relationship with the Ducks stage. But there was no significant difference in the expression of survivin, bFGF and VEGF in histological grade. Expression of bFGF and VEGF were found to be no significant difference in those patients whose tumor limited in the intestines when compared with the control group( P >0.05). While the tumor penetrated the intestines, the expression of bFGF and VEGF were found to be higher than that in the control group( P <0.001). The positive rate of bFGF and VEGF were higher in the node metastatic group when compared with the non-node metastatic group( P <0.0001). Conclusion The expression of survivin, bFGF and VEGF in colorectal carcinoma was related with clinic pathological parameters including lymphnode metastasis, Dukes staging and histological grade. It might be a biological index to evaluate the prognosis and biological behavior of colorectal carcinoma.