点阵Er:YAG激光照射兔口腔黏膜后的组织学变化

目的: 研究点阵Er:YAG激光照射口腔黏膜后的组织学变化。方法: 使用Er:YAG激光照射家兔口腔黏膜,应用H-E、Masson、弹性纤维染色方法,评估照射后1、2、4周黏膜上皮厚度、固有层胶原纤维和弹性纤维含量。采用SPSS19.0软件包对实验结果进行统计学分析。结果: 点阵Er:YAG照射后,黏膜损伤区仅限黏膜上皮内,3~7 d后完全愈合,不遗留色素及瘢痕,黏膜上皮厚度显著增加（P<0.05）,固有层胶原纤维、弹性纤维含量显著增加（P<0.05）。点阵Er:YAG激光照射家兔口腔黏膜损伤小、愈合快,黏膜上皮重构,黏膜固有层胶原变性、收缩。结论: 点阵Er:YAG激光照射口腔黏膜后,胶原纤维及弹性纤维增生、重组,黏膜收紧,弹性增加。

Histological change of rabbit oral mucosa after fractional Er:YAG laser irradiation

PURPOSE: Histological changes were studied to evaluate the effect of fractional Er:YAG laser treatment on oral mucosa tissues. METHODS: The rabbit oral mucosa was irradiated with Er:YAG laser. The epithelial thickness, lamina propria collagen fiber and elastic fiber content were assessed at 1, 2, 4 weeks after OP. H-E staining, Masson staining and elastic fiber staining were utilized. The experimental results were statistically analyzed by SPSS19.0 software package. RESULTS: After dot matrix Er:YAG irradiation, mucosal damage zone was limited only within the mucosal epithelium, and healed completely in 3-7 days, without leaving scar and pigment. The epithelial thickness was increased significantly (P<0.05), and the lamina propria collagen fiber and elastic fiber content were increased significantly (P< 0.05). After fractional Er:YAG laser irradiation, the rabbit had a small oral mucosa lesion and healed quickly. The epithelial remodeled. The mucosa lamina propria collagen contracted. CONCLUSIONS: After fractional Er:YAG laser irradiation, collagen fiber and elastic fiber proliferated and remodeled. The mucosa was tightening and the mucous membrane elasticity was increased.