波动氧诱导的新生大鼠视网膜病变模型

目的制备80%至10%的波动氧所致SD新生大鼠视网膜病变的模型。方法新生SD大鼠随机分成两组,模型组(n=12)置于氧箱中,80%至10%的波动氧24 h交替,持续7天后转入空气中饲养5天。对照组(n=12)置于空气中。应用腺苷二磷酸酶染色组织化学法进行视网膜铺片观察视网膜血管改变,眼球切片HE染色观察突破视网膜内界膜的内皮细胞核,免疫组织化学法检测视网膜血管内皮生长因子(VEGF)表达。对两组进行比较,判断波动氧诱导模型的可靠性。结果与对照组相比,模型组新生大鼠视网膜周边血管走形迂曲,神经节细胞排列紊乱,细胞数目减少。模型组视网膜内皮细胞核数目较对照组明显升高,差异有统计学意义[(31.2±6.2)比(1.5±1.0),P<0.01]。模型组VEGF在视网膜神经节层、内核层、外丛状层及色素细胞层均有强表达,对照组仅在神经节细胞层、内核层有较弱表达。结论吸入高幅波动氧可导致新生大鼠视网膜血管增生性病变,血管增生性视网膜组织中VEGF表达明显增强,证实80%至10%的波动氧诱导SD新生大鼠视网膜病变模型可靠。

Newborn rats model of oxygen fluctuations-induced retinopathy

Objective The authors developed a rat model of retinal neovascularization by repeated fluctuations of inhaled oxygen between hyperoxia(80%) to hypoxia(10%).MethodsTwenty-four newborn SD rats were randomly divided into oxygen and control groups.The control group was kept in room environment.The oxygen group was kept in the oxygen concentration that was alternated between 80% to 10% every 24 hours for 7 days.The status of retinal vasculature was observed after birth in both oxygen group and control group at 12 days.ADP enzymohistochemistry stain method of retinal stretched preparation was used to observe the change of retinal blood vessels.HE staining was used for counting the number of nuclei extending from retinal area into vitreous to identify extraretinal neovascularization.The immunohistochemistry method was used to measure the expression of VEGF in the retina.Results Compared with control group,the oxygen had abnormal changes in retinal vascular,the ganglion cells arranged abnormally with the decrease in number.The numbers of nuclei extending from the retinal area into the vitreous area in oxygen group were significantly higher compared with the control(P<0.001).The expression of VEGF in the oxygen group was significantly stronger than that of the control group.Conclusions High-amplitude fluctuations oxygen was associated with the development of retinal neovascularization in the retinopathy.VEGF expression was significantly increased in the oxygen fluctuations-induced neovascularized retina.The method was confirmed that SD rat model of retinopathy was induced by 80 to 10% oxygen fluctuations.