Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity |
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| Authors: | Tamm Natalia N Semenov Alexander G Seferian Karina R Bereznikova Anastasia V Murakami Maryann M Apple Fred S Koshkina Ekaterina V Krasnoselsky Mihail I Katrukha Alexey G |
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| Institution: | HyTest LTD, Intelligate, 6th floor, Joukahaisenkatu 6, 20520 Turku, Finland. |
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| Abstract: | ObjectivesTo compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays.Design and MethodsPlasma samples (n = 94) from HF patients were analyzed by the novel-type “Single Epitope Sandwich” (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli).ResultsThe SES assay measured 1.2- to 7.2-fold (2.1 ± 0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference.ConclusionsThe SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms. |
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