PTEN和mTOR信号转导通路在胆管癌发展中作用的研究

目的: 研究PI3K/PTEN/AKT/mTOR信号转导通路中mTOR和PTEN蛋白在胆管癌中的表达及其在胆管癌发生、发展中的作用。方法:用免疫组织化学方法和RT-PCR法，检测胆管癌中mTOR 和PTEN 的表达。结果:与正常组织相比，免疫组化法和RT-PCR两种方法结果均显示，胆管癌中的mTOR表达明显增加，而PTEN的表达明显下降;两者呈负相关（r=-0.862,P<0.01）。结论:PI3K/PTEN/AKT/mTOR信号转导通路中重要的调节位点和节点PTEN在胆管癌中的表达明显降低，而mTOR的表达明显增高。〖KG(*8〗提示该信号转导通路在介导胆管癌的发生、发展的过程中起重要作用。

Effect of expression of PTEN and mTOR Gene Protein in the development of cholangiocarcinoma

Objective To explore the expression and relationship of PTEN and mTOR in the development of cholangiocarcinoma, determine the effect of the PI3K/PTEN/AKT/mTOR signal transduction on the development of cholangiocarcinoma. Methods The expression of roTOR and PTEN in the cholangiocarcinoma was detected by the immunohistochemistry and RT-PCR method. Results Compared to normal tissues, the expression of roTOR gene in cholangiocarcinoma significantly increased, but the expression of PTEN gene decreased. There was a negative correlation between roTOR gene and PTEN gene expression in cholangiocarcinoma. Conclusions The expression of roTOR gene in cholangiocarcinoma was increased and the expression of PTEN was decreased. It suggested that the mTOR gene and PTEN gene could play an important role in the process of development of cholangiocarcinoma.