地钱愈伤组织的诱导及其细胞培养条件的建立

目的探计对地钱愈伤组织的诱导和悬浮细胞培养的条件。方法用MSK 2和MS培养基对地钱配子体进行愈伤组织诱导，运用模糊评判对MSK-2培养基生长激素组合进行综合性状评价。结果MSK-2和Ms（2mg/L 6 BA）均能诱导出地钱愈伤组织，其中MSK 2的诱导效果较好。光照（3000～8000 lux）能有效地促进地钱愈伤组织生长。激素配比组合是0.5mg/L 2,4 D+2mg/L NAA+2mg/L 6-BA适合于地钱细胞生长以及次生代谢生产。 结论地钱愈伤组织的诱导成功和悬浮细胞培养条件的建立，为探索药用苔藓植物次生代谢产物生产提供新的途径以及对苔藓植物细胞规模化培养提供新的理论指导。

Initiation of callus tissues and establishment of suspension culture of Marchantia polymorpha L. cells

Objective To induce callus tissues and establish suspension culture of Marchantia polymorpha L.Methods MSK-2 and MS mediums were used to induce gametophytes into callus tissues,and the fuzzy comprehensive evaluation method was used to assess the optimal phytohormone combination.Results Both MSK-2 and MS(2 mg/L 6-BA) mediums could induce callus tissue formation,and the MSK-2 medium was superior to the MS medium for callus tissue induction.Light intensity(3 000-8 000 lux) effectively promoted the callus tissue growth.Optimal phytohormone combination for cell growth and secondary metabolism production was 0.5 mg/L 2,4-D 2 mg/L NAA 2 mg/L 6-BA.Conclusions Successful induction of the callus tissues and establishment of suspension culture of Marchantia polymorpha L.provide a new pathway for exploring the pharmaceutical moss to produce secondary metabolism and new theory guidance for suspension culture of moss cells on a large scale.