Binding of enterotoxigenicEscherichia coliexpressing different colonization factors to tissue-cultured Caco-2 cells and to isolated human enterocytes |
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| Institution: | 1. Department of Medical Microbiology and Immunology, Göteborg University, Guldhedsgatan 10, S-41346 Göteborg, Sweden;2. Instituto Nacional de Microbiolog?́a “Dr. Carlos G. Malbrán” Vélez Sarsfield 563, 1281, Buenos Aires, Argentina;1. Laboratory of Behavioral Neuroscience, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA;2. Section of Biomedical Image Analysis, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA;3. Translational Gerontology Branch, Longitudinal Studies Section, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA;1. Institute of Medical Microbiology, Jena University Hospital, Jena, Germany;2. Institute of Physiological Chemistry and Pathobiochemistry, Germany;3. Cells-in-Motion Cluster of Excellence, University of Muenster, Muenster, Germany;4. Institute of Medical Microbiology, University Hospital of Muenster, Muenster, Germany;5. Surgery, Orthopedics and Traumatology, Special Trauma Surgery, Clinic of Weimar, Germany;6. Institute of Hygiene, University Hospital Muenster, Muenster, Germany;7. Department of Translational Dermatoinfectiology, University of Muenster, Muenster and Department of Dermatology, University Hospital of Halle, Halle, Germany;1. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo—Escola Paulista de Medicina, São Paulo, Brazil;2. Department of Microbiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan;3. Department of Infectious Diseases, Division of Microbiology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan;4. Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan;5. Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde—Ilha do Fundão, Rio de Janeiro, Brazil;6. Seção de Bacteriologia, Instituto Adolfo Lutz, Secretaria de Estado da Saúde, São Paulo, Brazil;1. Key Laboratory of In-situ Property-improving Mining of Ministry of Education, Taiyuan University of Technology, Taiyuan, 030024, China;2. Department of Mining Engineering, Taiyuan University of Technology, Taiyuan, 030024, China;1. Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China;2. Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China;3. Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China |
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| Abstract: | The binding of ETEC strains expressing different colonization factors to the human enterocyte-like cell line Caco-2 and to isolated human enterocytes were determined. Strains expressing CFA/I, CS2, CS4+CS6, CS5+CS6, CS7, CFA/III+CS6 and PCFO166 adhered well to both types of cells whereas bacteria producing CS1, CS6 only, or CS17 did not adhere to either Caco-2 cells or to jejunal human enterocytes, suggesting that similar binding structures are present in both cell types. However, in some instances, binding of bacteria to the two types of cells differed, e.g. bacteria expressing CS3 or PCFO9 bound well to human enterocytes but not to Caco-2 cells. Conversely, bacteria producing PCFO20 or PCFO159 only adhered to Caco-2 cells and not to jejunal enterocytes. With few exceptions, this inability to bind to human enterocytes was the same for cells from all parts of the small intestine. This study contradicts previous reports suggesting that the binding structures of Caco-2 cells are identical to those of human enterocytes. |
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