HBV病毒蛋白在C57-TgN(HBVadr2.0)SMMU小鼠中的表达

目的:分析C57-TgN(HBVadr2.0)SMMU"3号"品系转基因小鼠血清和组织中病毒蛋白的表达及其特征.方法:以138只F6～F17代C57-TgN(HBVadr2.0)SMMU"3号"品系的转基因小鼠为研究对象,采用血清ELISA检测和免疫组织化学的方法分析病毒蛋白在转基因小鼠中的表达.结果:转基因小鼠血清中HBsAg和HBeAg的阳性率分别为55.18%和25.00%,血清中HBsAg的出现时间多在3个月龄之前(占93.33%),且持续时间在9个月以上(占95.56%),个体之间具有一定差异.转基因小鼠肝组织中至少可检测到一种病毒蛋白,HBsAg、HBcAg和X蛋白的检出率分别为85.82%、58.24%和49.61%.HBsAg分布于肝细胞质中,HBcAg和X蛋白既可分布于肝细胞核中,也可分布干肝细胞质中.HBsAg的表达具有细胞特异性(门管区周围的肝细胞)和组织特异性(肝和肾),X蛋白除在肝脏和肾脏中表达外,还可在脑组织中表达.结论:C57-TgN(HBVadr2.0)SMMU"3号"品系转基因小鼠血清和肝肾等组织中有病毒蛋白的表达,病毒蛋白的分布具有组织细胞特异性和个体差异.

Expression of hepatitis B virus proteins in HBV transgenic mice strain C57-TgN(HBVadr2.0)SMMU

Objective:To evaluate expression of hepatitis B virus proteins in serum and tissues of C57-TgN(HBVadr2.0)SMMU3 transgenic mouse. Methods: Totally 138 transgenic mice of C57-TgN(HBVadr2.0)SMMU3 strain F6-F17 offspring were used to estimate the expression of hepatitis B virus gene by ELISA and immunohistochemistry. Results: HBsAg and HBeAg were expressed in the serum of 55.18% and 25.00% transgenic mice,respectively. Appearance time of serum HBsAg was within 3 months in 93.33% mice and persisted for more than 9 months in most mice(95.56%)with individual difference. HBsAg, HBcAg and X protein were expressed in liver tissue of 85.82%, 58.24% and 49.61% transgenic mice,respectively. Distribution of HBsAg was mainly in cytoplasm of hepatocyte.Furthermore, HBcAg and X protein were expressed in cytoplasm and/or nucleus of hepatocyte. Cell-specific and tissue-specific expression of HBsAg was detected mainly in the hepatocyte near portal area of liver and kidney. Distribution of X protein was found in the liver, kidney and brain. Conclusion: C57-TgN(HBVadr2.0)SMMU3 transgenic mice express hepatitis B virus proteins in the serum, liver and kidney. The expression is cell-specific and tissue-specific, but has individual difference.