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| 雷公藤内酯醇降调胰腺癌细胞5-脂氧合酶表达作用研究 | |
| 引用本文： | 吴深宝,周国雄,张弘,黄介飞,马鹏程,鄂群,魏群,丁晓凌.雷公藤内酯醇降调胰腺癌细胞5-脂氧合酶表达作用研究[J].医药导报,2007,26(5):454-457. |
| 作者姓名： | 吴深宝 周国雄 张弘 黄介飞 马鹏程 鄂群 魏群 丁晓凌 |
| 作者单位： | 1. 南通大学附属医院消化内科,226001 2. 中国医学科学院、中国协和医科大学皮肤病研究所,南京,210000 3. 南通大学医学院病理教研室,226001 |
| 摘 要： | 目的探讨雷公藤内酯醇（TL）对胰腺癌细胞中5 脂氧合酶(5 LOX)蛋白表达及胰腺癌细胞凋亡的影响。方法应用RT PCR法和免疫细胞化学法检测胰腺癌细胞株SW1990中5 LOX表达，同时应用流式细胞术检测胰腺癌细胞SW1990的凋亡指数。结果胰腺癌细胞株SW1990表达5 LOX，正常胰腺组织不表达； TL可抑制SW1990中5 LOX表达，并促进胰腺癌细胞SW1990凋亡，作用随浓度的增加而增强(P＜0.01)；细胞凋亡与5 LOX蛋白的表达相关(P＜0.01)。结论TL可抑制胰腺癌细胞SW1990中5 LOX的表达，并促进胰腺癌细胞凋亡 |
| 关 键 词： | 雷公藤内酯醇 胰腺肿瘤 5-脂氧合酶 凋亡 |
| 文章编号： | 1004-0781（2007）05-0454-03 |
| 收稿时间： | 2006-09-11 |
| 修稿时间： | 2006-11-03 |
Down-regulation of 5-lipoxygenase Expression in Pancreatic Cancer Cell by Triptolide |
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| WU Shen-bao,ZHOU Guo-xiong,ZHANG Hong,HUANG Jie-fei,MA Peng-cheng,E Qun,WEI Qun,DING Xiao-ling.Down-regulation of 5-lipoxygenase Expression in Pancreatic Cancer Cell by Triptolide[J].Herald of Medicine,2007,26(5):454-457. | |
| Authors: | WU Shen-bao ZHOU Guo-xiong ZHANG Hong HUANG Jie-fei MA Peng-cheng E Qun WEI Qun DING Xiao-ling |
| Abstract: | Objective To investigate the effect of Triptolide on 5-lipoxygenase(5-LOX) expression and apoptosis of human pancreatic cancer cell. Methods The immunocytochemical staining and RT-PCR were employed to detect the expression of 5-LOX in the pancreatic cell line SW1990.The apoptotic index of SW1990 was determined by flow cytometry(FCM). Results The pancreatic cell line SW1990 expressed 5-LOX,but the normal pancreatic cells did not.TL inhibited 5-LOX expression and promoted SW1990 cell apoptosis in a dose dependent manner(P<0.01).The cell apoptosis was correlated to the expression of 5-LOX(P<0.01). Conclusion TL is able to inhibit 5-LOX expression and facilitate the cell apoptosis in pancreatic cancer cell line SW1990. |
| Keywords: | Triptolide Pancreatic neoplasm 5-lipoxygenase Apoptosis |
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