无血清饥饿法去除神经组织培养中的成纤维细胞

介绍去血清饥饿培养方法去除神经组织培养中的成纤维细胞。取 2日龄大鼠双侧坐骨神经和双侧嗅球 ,酶消化、离心 ,1 0 %胎牛血清 (FCS)DMEM体外培养 ,如果成纤维细胞生长迅速 ,换无血清DMEM培养液培养。根据细胞生长状况可反复上述过程。S 1 0 0免疫荧光染色鉴定雪旺细胞和嗅鞘细胞纯度。结果 :换无血清培养液后 ,成纤维细胞迅速死亡 ,S 1 0 0免疫荧光染色雪旺细胞和嗅鞘细胞的纯度可达 98%～ 99%。此方法简捷、易操作 ,为神经组织培养中去成纤维细胞的一种好方法。

Free-Serum Culture Method to Remove Fibroblast in Nerve Tissue

The free-serum culture method was presented to remove fibroblast in nerve tissue. Sciatic nerves and olfactory bulbs from 2 d old rats were dissected, treated with trypsin and collagenase, followed by centrifugation and cultured in DMEM with 10% foetal calf serum. If fibroblast rapidly multiplied to form a confluent monolayer, the media was changed into free-serum DMEM. The process was done again. S-100 immunofluorescent staining was used to identify the purity of Schwann cells and olfactory ensheath cells. Results showed that fibroblast rapidly died in free-serum media, the purity of Schwann cells and olfactory ensheath cells were up to 98%～99% using immunofluorescent staining. The findings prove that it is a good method to remove fibroblast in nerve tissue culture because the process is simple and is operated easily.