染锰大鼠血清NO、NOS、SOD的变化

为探讨染锰大鼠血清中一氧化氮（ＮＯ）浓度、一氧化氮合酶（ＮＯＳ）活力及超氧化物歧化酶（ＳＯＤ）活力的变化。采用饮水染毒建立动物模型；血清ＮＯ浓度测定采用硝酸还原酶法、血清ＮＯＳ活性测定采用ＮＯＳ催化Ｌ－Ａｒｇ法，血清ＳＯＤ活力测定业硝酸盐显色法；采用ＳＡＳ６．１２统计软件作测定结果均数的ｔ检验。结果显示染锰组ＮＯＳ活力显著低于对照组（Ｐ〈０．０５），而ＮＯ浓度和ＳＯＤ活力染锰组和对照组无显著性差异

Changes of Serum Nitric Oxide,Nitric Oxide Synthase and Superoxide Dismutase in Manganese Exposed Rats

To study the changes of serum nitric oxide(NO) concentration,bioactivities of nitric oxide synthase(NOS) and superoxide dismutase(SOD)in manganese exposed rats,animal models were made by drinking manganese chloride solution(1 0 g/L).Serum NO was determined by nitric oxide reductase method.NOS was determined by NOS catalyzed L Arg method,and SOD by nitrate colorimetric method.Data obtained were analyzed by SAS 6 12 statistic software for t test.Results showed that the bioactivity of NOS in the manganese group was significantly lower than that of the control(P<0 05),however,no significant difference of NO and SOD existed in the serum of two groups(P>0 05).It indicatcd that the bioactivity of serum NOS could be lowered by manganese exposure.But the changes of serum NO and SOD needs further confirmation.