Cultivation of bloodstream forms of Trypanosoma carassii, a common parasite of freshwater fish |
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| Authors: | Peter Overath Jens Ruoff York-Dieter Stierhof Jochen Haag Herbert Tichy Iva Dyková Jiri Lom |
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| Institution: | (1) Max-Planck-Institut für Biologie, Abteilung Membranbiochemie, Corrensstrasse 38, D-72076 Tübingen, Germany Tel.: +49-7071-601236; Fax: +49-7071-62971; E-mail: peter.overath@tuebingen.mpg.de, DE;(2) Max-Planck-Institut für Biologie, Abteilung Immungenetik, Corrensstrasse 40, D-72076 Tübingen, Germany, DE;(3) Institute of Parasitology, Academy of Sciences of the Czech Republic, Branišovska 31, 37005 Ceské Budejovice, Czech Republic, |
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| Abstract: | Trypanosoma carassii (syn. T. danilewskyi) is a widespread parasite of carp and other cyprinid as well as some noncyprinid freshwater fish. It lives extracellularly
in the blood and tissues of its hosts, causing chronic infections. In this paper the isolation of T. carassii from fish blood and the propagation and cloning of bloodstream forms in vitro are described. By several criteria, cultured
and fish-derived trypomastigotes are indistinguishable. The culture system should be useful for the biochemical characterization
of this trypanosome and its interaction with the fish immune system.
Received: 10 September 1997 / Accepted: 26 November 1997 |
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