HepaSphere微球联合Avasting栓塞治疗VX2兔肝癌的实验研究

目的 探讨HepaSphere微球联合Avasting肝动脉栓塞治疗兔移植性肝癌的疗效.方法 建立40只兔VX2肝癌模型,随机分为四组,每组10只,A组：肝动脉灌注生理盐水;B组：肝动脉灌注Avasting;C组：肝动脉HepaSphere栓塞;D组：肝动脉HepaSphere＋Avasting栓塞.两周后复查CT确定种植成功并计算瘤体大小,免疫组化方法检测肝肿瘤血管内皮生长因子（VEGF）的表达和肿瘤微血管密度（MVD）.所得数据使用SPSS12.0统计软件进行处理,各组间比较采用q检验、x2检验.结果 肿瘤生长抑制D组明显优于其他组（P＜0.05）; VEGF的表达及MVD计数D组显著低于其他组（P＜0.05）.结论 HepaSphere微球联合Aasting行兔肝动脉栓塞能抑制移植型肝癌的生长及肝内转移,并对肿瘤组织的微血管和VEGF的表达具有显著的抑制作用.

Effect of transarterial embolization with Avasting combined with HepaSphere in the treatment of rabbit with VX2 hepatic carcinoma

Objective To study the therapeutic effect of transarterial embolization with Avasting and hepa- Sphere for rabbits with VX2 liver carcinoma. Methods Forty rabbits implanted with liver VX2 tumors were random- ly divided into four groups, with 10 rabbits in each group. All rabbits were demonstrated with liver neoplasmas by MSCT and treated with transhepatic arterial embolization under the open laparotomy. Group A was given 3 ml saline; Group B was infused with 5 mg Avasting injection; Group C was embolized with hepaSphere; Group D was adminis- trated with 5 mg Avasting and hepaSphere. Mutislice spiral CT scan was performed to measure tumor volume and the expression of MVD and VEGF were determined with immunohistochemistry on the 14 ~ day after treatment. All param- eter thus gained were compared among the four groups. Results Group D showed the growth of tumor significantly better other three groups （P〈0.05）. The expression of MVD and VEGF in group D was significantly lower than that in other groups （P〈0.05）. Conclusion Transarterial embolization with Avasting combined hepaSphere can effectively in- hibit the growth of hepatic tumor and reduce the expression level of VEGF and the formation of the tumor vessels.